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OALib Journal期刊
ISSN: 2333-9721
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睾丸酮丛毛单胞菌teir基因插入失活突变株构建及降解特性分析
DOI: 10.3724/SP.J.1145.2009.00830, PP. 830-834
Keywords: 睾丸酮丛毛单胞菌,teir基因,同源重组,降解酶3a-hsd/cr类固醇,类固醇
Abstract:
睾丸酮丛毛单胞菌（comamonastestosteroni）是革兰氏阴性菌，能够以各种类固醇化合物作为唯一碳源和能源，生物信息学分析发现睾丸酮丛毛单胞菌teir基因编码蛋白属于luxr-type转录调控蛋白.利用同源重组原理构建teir基因插入失活突变体c.mut，初步研究了teir基因在类固醇代谢途径中的作用，以及对睾丸酮丛毛单胞菌关键降解酶3a-hsd/cr的表达影响.结果发现，teir基因插入失活突变体c.mut在lb培养基中的生长速率不受影响，突变体c.mut抑制了teir基因和关键降解酶3a-hsd/cr的表达，同时突变体c.mut丧失了以睾丸酮作为碳源进行生长的能力.结果表明teir基因调节睾丸酮丛毛单胞菌类固醇代谢，同时teir基因表达对3a-hsd/cr基因的表达是必需的.图7参14
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