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OALib Journal期刊
ISSN: 2333-9721
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鞑靼荞麦离体再生体系的建立
DOI: 10.3724/SP.J.1145.2009.00786, PP. 786-789
Keywords: 鞑靼荞麦,苦荞麦,组织培养,愈伤组织,离体再生,植物生长调节剂
Abstract:
研究了苗龄、外植体、激素配比对鞑靼荞麦（fagopyrumtataricumgaertn.）离体培养的影响，初步建立了鞑靼荞麦离体再生体系.结果表明，鞑靼荞麦离体再生的最佳取材时间为苗龄6~8d；诱导愈伤组织的最适培养基为ms＋2.0mg/l2,4-d＋1.5mg/l6-ba，子叶诱愈率达75%左右，下胚轴诱愈率可高达86.62%；愈伤组织分化的最适培养基为ms+0.1mg/liaa＋2.0mg/l6-ba＋1.0mg/lkt＋0.5mg/ltdz，来自下胚轴愈伤组织的分化率可达9.52%.下胚轴的诱愈率与分化率均高于子叶，更适于离体再生培养.培养基中加入agno3后，能有效降低褐化率.生根最适培养基为含有0.5mg/lnaa的1/2ms培养基，生根率在50%左右.tdz在诱导鞑靼荞麦的愈伤组织分化出芽的过程中起到明显的促进作用，可提高分化率约20%.表3图2参23
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