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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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松毛虫遗传多样性研究中aflp反应体系的建立
DOI: 10.3724/SP.J.1145.2009.00875, PP. 875-879
Keywords: 松毛虫,aflp,反应体系,引物筛选,遗传多样性
Abstract:
以油松毛虫为材料，对t4dna连接酶不同用量、预扩增中的mg2+浓度、dntp浓度、引物浓度以及选择性扩增中的预扩增产物稀释倍数、mg2+浓度、dntp浓度、引物浓度和taq酶浓度进行了比较分析.最终建立了适合松毛虫的aflp反应体系.用优化的aflp反应体系，以油松毛虫、赤松毛虫和马尾松毛虫为材料筛选引物，从81对引物组合中筛选出10对多态性高的引物组合.图9参17
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