OALib Journal期刊
ISSN: 2333-9721
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拮抗菌b96-ii对芦笋枯萎菌的抑菌作用
DOI: 10.3724/SP.J.1145.2009.00861, PP. 861-865
Keywords: 芦笋枯萎菌,拮抗菌,溶菌作用,电导率,总溶解性固体,细胞膜
Abstract:
为明确拮抗菌b96-ii对芦笋枯萎菌的抑制效果并探明其抑菌特性及方式,对经b96-ii处理后芦笋枯萎菌的生长速率、液体电解质、电导率及菌丝重量等进行了测定研究.结果表明,在107~108cfu/ml时,b96-ii对芦笋枯萎菌均有显著的抑制效果.连续3wk室内观察发现,b96-ii对芦笋枯萎菌丝有持续的抑制作用,与对照比较抑制率为94.07%~88.98%.田间防治效果为93.40%,而农药多菌灵处理的防治效果仅为65.12%.拮抗菌b96-ii对芦笋枯萎菌的抑制方式主要为:(1)对孢子形成的抑制作用;(2)对孢子的溶解作用;(3)对菌丝生长的抑制作用;(4)对菌丝的致畸作用;(5)对菌体细胞的破损作用.b96-ii处理后36h,溶液总溶解性固体和电导率增高,而菌丝重量下降.图4表3参14
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