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OALib Journal期刊
ISSN: 2333-9721
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海洋破囊壶菌△5-延长酶和△4-脱饱和酶在酿酒酵母中的共表达
DOI: 10.3724/SP.J.1145.2009.00851, PP. 851-855
Keywords: 破囊壶菌,二十二碳六烯酸,延长酶,脱饱和酶,共表达
Abstract:
二十二碳六烯酸（docosahexaenoicacid，dhac22:6n-3）是具有各种重要生理功能的高度不饱和脂肪酸.分别以质粒pytfd5和pyfad4为模板，扩增获得860bp的△5-延长酶基因（elo5）和1600bp的△4-脱饱和酶基因（fad4）.利用重叠延伸pcr构建elo5-fad4融合基因，hindiii/sphi双酶切后连接到经同样处理过的pyes2.0载体，构建重组表达质粒pyelo5-fad4.转化酿酒酵母尿嘧啶缺陷型菌株invscl，通过缺少尿嘧啶的选择性培养基筛选阳性克隆子.添加外源脂肪酸c20:5底物，半乳糖诱导表达.气相色谱？质谱分析表明，重组酵母总脂肪酸中出现了dha（二十二碳六烯酸，c22:6n-3）新产物，融合基因elo5-fad4在酿酒酵母中得到了表达.图7表1参18
| [1] | 4passorns,laotengk,rachadawongs,tanticharoenm,cheevadhanaraks.heterologousexpressionofmucorrouxiidelta(12)-desaturasegeneinsaccharomycescerevisiae.biochembiophysrescommun,1999,263(1):47~~51
|
| [2] | 5sakuradanie,shimizus.genecloningandfunctionalanalysisofaseconddelta6-fattyaciddesaturasefromanarachidonicacid-producingmortierellafungus.bioscibiotechnolbiochem,2003,67(4):704~711
|
| [3] | 8jiangxz(江贤章),qinln(秦丽娜),tianby(田宝玉),shuzy(舒正玉),huangjz(黄建忠).cloningandexpressionoftwoelongasegenesinvolvedinthebiosynthesisofdocosahexaenoicacidinthraustochytriumsp.fjn-10.actamicrobiolsin(微生物学报),2008,48(2):176~183
|
| [4] | 9liulx(刘丽霞),jiangxz(江贤章),qinwx(覃文新),yaogf(姚根富),huangjz(黄建忠).identificationofthe△4fattyaciddesaturasegenefromthraustochytriumsp.fjn-10.indmicrobiol(工业微生物),2006,36(1):1~6
|
| [5] | 12zhengy(郑耘),lihp(李华平),xiaohg(肖火根),fanhz(范怀忠).constructionofplantexpressionvectoroffusiongeneswithbananabunchytopvirusreplicaseandcucumbermosaicviruscoatprotein.jsouthchinaagricuniv(华南农业大学学报),2005,26(3):18~21
|
| [6] | 13qiux,hongh,mackenziesl.identificationofadelta4fattyaciddesaturasefromthraustochytriumsp.involvedinthebiosynthesisofdocosahexanoicacidbyheterologousexpressioninsaccharomycescerevisiaeandbrassicajuncea.jbiolchem,2001,276(34):31561~31566
|
| [7] | 1rinaldib,dipierrop,vitellimr,d’amicom,berrinol,rossif,filippellia..effectsofdocosahexaenoicacidoncalciumpathwayinadultratcardiomyocytes.lifesci,2002,71(9):993~1004
|
| [8] | 2sijtsmal,deswaafme.biotechnologicalproductionandapplicationsoftheω-3polyunsaturatedfattyaciddocosahexaenoicacid.applmicrobiolbiotechnol,2004,64(2):146~153
|
| [9] | 3certikm,shimizus.biosynthesisandregulationofmicrobialpolyunsaturatedfattyacidproduction.jbioscibioeng,1999,87(1):1~14
|
| [10] | 6schneiterr,tatzerv,goggg,leitnere,kohlweinsd.elo1p-dependentcarboxy-terminalelongationofc141delta(9)toc16:1delta(11)fattyacidsinsaccharomycescerevisiae.jbacteriol,2000,182(13):3655~3660
|
| [11] | 7huangjz(黄建忠),liulx(刘丽霞),wusg(吴松刚).profileoffattyacidsandanalysisof18srdnasequenceinthraustochytriumsp.fjn-10highlyproducingdha.pharmbiotechnol(药物生物技术),2005,12(1):1~5
|
| [12] | 10kozakm.recognitionofaugandalternativeinitiatorcodonsisaugmentedbyginposition+4butisnotgenerallyaffectedbythenucleotidesinpositions+5and+6.emboj,1997,16(9):2482~2492
|
| [13] | 11kozakm.compilationandanalysisofsequencesupstreamfromthetranslationalstartsiteineukaryoticmrnas.nucleicacidsres,1984,12(2):857~872
|
| [14] | 14huangjz(黄建忠),jiangxz(江贤章).isolationofschizochytriumsp.fju-512withhighyieldofdhaandcomparativeanalysisonits18srrnagenesequence.chinjapplenvironbiol(应用与环境生物学报),2005,11(2):202~207
|
| [15] | 15sayanovao,haslamr,qib,lazaruscm,napierja.thealternativepathwayc20δ8-desaturasefromthenon-photosyntheticorganismacanthamoebacastellaniiisanatypicalcytochromeb5-fusiondesaturase.febslett,2006,580(8):1946~1952
|
| [16] | 16beaudoinf,michaelsonlv,heysj,lewismj,shewrypr,sayanovao,napierja.heterologousreconstitutioninyeastofthepolyunsaturatedfattyacidbiosyntheticpathway.procnatlacadsciusa,2000,97(12):6421~6426
|
| [17] | 17pereirasl,leonardae,huangys,chuanglt,mukerjip.identificationoftwonovelmicroalgalenzymesinvolvedintheconversionoftheω3-fattyacid,eicosapentaenoicacid,intodocosahexaenoicacid.biochemj,2004,384(2):357~366
|
| [18] | 18domerguef,abbadia,zähringeru,moreauh,heinze.invivocharacterizationofthefirstacyl-coaδ6-desaturasefromamemberoftheplantkingdom,themicroalgaostreococcustauri.biochemj,2005,389(2):483~490
|
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