|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
一种嗜热细菌来源角质酶的分离纯化及酶学性质
DOI: 10.3724/SP.J.1145.2009.00846, PP. 846-850
Keywords: 嗜热细菌,角质酶,分离纯化,酶学性质,热稳定性
Abstract:
通过跟踪发酵液中pnpb水解酶活性，对角质诱导的thermobifidafusca发酵液进行分离纯化.采用活性炭脱色、硫铵沉淀、phenylhp疏水色谱、deaesepharose阴离子交换色谱等方法，分离纯化得到电泳纯pnpb水解酶.该酶水解角质可得到角质单体，是一种角质酶.sds-page电泳结果显示，角质酶表观分子量约为29×103.该酶的最适温度为60℃，在40℃和60℃下均具有良好的热稳定性.最适ph为8.0，ph稳定范围为6.0~9.0.该角质酶的生化性质适合在纺织工业中应用.图8表2参17
| [1] | 1egmondmr,devliegj.fusariumsolanipisicutinase.biochimie,2000,82:1015~1021
|
| [2] | 2nardinim,dijkstrabw.alpha/betahydrolasefoldenzymes:thefamilykeepsgrowing.curropinstructbiol,1999,9:732~737
|
| [3] | 3vertommenma,nierstraszva,veerm,warmoeskerkenmm.enzymaticsurfacemodificationofpoly(ethyleneterephthalate).jbiotechnol,2005,120:376~386
|
| [4] | 4deganio,gepsteins,dosoretzcg.potentialuseofcutinaseinenzymaticscouringofcottonfibercuticle.applbiochembiotechnol,2002,102~103:277~289
|
| [5] | 5purdyre,kolattukudype.depolymerizationofahydroxyfattyacidbiopolymer,cutin,byanextracellularenzymefromfusariumsolanif.pisi:isolationandsomepropertiesoftheenzyme.archbiochembiophys,1973,159:61~69
|
| [6] | 6wanggy,michailidestj,hammockbd,leeym,bostockrm.affinitypurificationandcharacterizationofacutinasefromthefungalplantpathogenmoniliniafructicola(wint.)honey.archbiochembiophys,2000,382:31~38
|
| [7] | 7wanggy,michailidestj,hammockbd,leeym,bostockrm.molecularcloning,characterization,andexpressionofaredox-responsivecutinasefrommoniliniafructicola(wint.)honey.fungalgenetbiol,2002,35:261~276
|
| [8] | 8bifz(毕凤珍),shijl(师俊玲),liy(李寅).cutinaseproductionconditionswiththermobifidafusca.chinjapplenvironbiol(应用与环境生物学报),2005,11(5):608~610
|
| [9] | 9zhangsl(张守亮),chenj(陈坚),huazz(华兆哲).mutagenesisofcutinase-producingstrainthermobifidafuscawsh03-11andoptimizationoffermentationconditionsofmutantwithhighcutinaseproduction.chemind&enginproc(化工进展),2006,25:533~537
|
| [10] | 10cunhamt,costamj,caladocr,fonsecalp,aires-barrosmr,cabraljm.integrationofproductionandaqueoustwo-phasesystemsextractionofextracellularfusariumsolanipisicutinasefusionproteins.jbiotechnol,2003,100:55~64
|
| [11] | 11ferreirabs,caladocr,vankeulenf,fonsecalp,cabraljm,dafonsecamm.towardsacosteffectivestrategyforcutinaseproductionbyarecombinantsaccharomycescerevisiae:strainphysiologicalaspects.applmicrobiolbiotechnol,2003,61:69~76
|
| [12] | 12longhis,cambillauc.structure-activityofcutinase,asmalllipolyticenzyme.biochimbiophysacta,1999,1441:185~196
|
| [13] | 13sebastianj,chandraak,kolattukudype.discoveryofacutinase-producingpseudomonassp.cohabitingwithanapparentlynitrogen-fixingcorynebacteriumsp.inthephyllosphere.jbacteriol,1987,169:131~136
|
| [14] | 14gindrok,pezetr.purificationandcharacterizationofa40.8-kdacutinaseinungerminatedconidiaofbotrytiscinereapers.:fr.femsmicrobiollett,1999,171:239~243
|
| [15] | 15alischmm,herrmanna,zimmermannw.increaseofthehydrophilicityofpolyethyleneterephthalatefibresbyhydrolasesfromthermomonosporafuscaandfusariumsolanif.sp.pisi.biotechnollett,2006,28:681~685
|
| [16] | 16osmansf,irwinp,fettwf,connorjv,parrisn.preparation,isolation,andcharacterizationofcutinmonomersandoligomersfromtomatopeels.jagricfoodchem,1999,47:799~802
|
| [17] | 17waltontj,kolattukudype.determinationofthestructuresofcutinmonomersbyanoveldepolymerizationprocedureandcombinedgaschromatographyandmassspectrometry.biochemistry,1972,11:1885~1896
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
