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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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黑曲霉β-葡萄糖苷酶的筛选、克隆及表达
DOI: 10.3724/SP.J.1145.2009.00423, PP. 423-426
Keywords: 黑曲霉,β-葡萄糖苷酶,克隆,rt-pcr,毕赤酵母,表达
Abstract:
摘要从曲霉属10株试验菌中筛选得到一株产β-葡萄糖苷酶活性较高的黑曲霉fta-008.该菌株在适宜的培养条件下，β-葡萄糖苷酶的最高活性达2.89u/ml，适宜产酶周期为3d.通过设计特异引物，以该菌株的总rna为模板，采用rt-pcr技术获得大小为2511bp的cdna片段，该序列与genbank中aj132386的β-葡萄糖苷酶序列相比，氨基酸序列同源性达96.8%.经毕赤酵母表达，β-葡萄糖苷酶比活力达7.85u/mg.图3表1参13
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