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OALib Journal期刊
ISSN: 2333-9721
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草鱼肠道纤维素酶产生菌主要种类的分离与鉴定
DOI: 10.3724/SP.J.1145.2009.00414, PP. 414-418
Keywords: 草鱼,肠道,纤维素酶产生菌,分离,鉴定
Abstract:
摘要从我国特有的草鱼肠道内分离到纤维素酶产生菌，其中4株具有较高的纤维素分解能力，测量了其羧甲基纤维素酶(cmcase)、棉花酶和滤纸酶(fpase)活性，并进行了种类鉴定.结果表明，草鱼肠道具有一定的纤维素分解能力，可能来自于肠道细菌所产生的外源性纤维素酶的协同作用；草鱼肠道内纤维素分解菌具有较高酶活性；不同菌株所产生的相同纤维素酶的活性存在差异；其中，x7的cmcase活性最高，达0.83u/ml，x5的棉花酶活性最高，达0.87u/ml，x8的fpase活性最高，达0.54u/ml.4种菌株的菌落形态有差异，经16srrna基因鉴定，x5、x6、x7为枯草芽胞杆菌(bacillussubtilis)，x8为阿氏肠杆菌(enterobacterasburiae).图6表3参19
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