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OALib Journal期刊
ISSN: 2333-9721
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一株产木聚糖酶的耐冷皮壳正青霉菌鉴定与酶谱分析
DOI: 10.3724/SP.J.1145.2009.00376, PP. 376-379
Keywords: 木聚糖酶,耐冷菌,皮壳正青霉,酶谱分析
Abstract:
摘要从青海冻土中分离得到1株产木聚糖酶的青霉菌qh11，通过菌落特征观察、显微特征观察和18srdna同源性序列分析，鉴定该菌为皮壳正青霉，其最适生长温度为20℃.该菌以玉米芯水不溶木聚糖为碳源，于20℃摇瓶培养，d4木聚糖酶达到高峰，酶活为28.13u/ml，比酶活为9.73u/mg，粗酶液的最适温度为47.5℃.sds-page和酶谱分析表明，该菌可产生3种木聚糖酶，其亚基相对分子质量(mr)分别为20.5×103、23.0×103和35.0×103.图6参15
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