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OALib Journal期刊
ISSN: 2333-9721
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发光细菌生物活性的调控方法
DOI: 10.3724/SP.J.1145.2011.00264, PP. 264-267
Keywords: 发光细菌,保护剂,冷冻干燥法,在线毒性监测仪
Abstract:
为维持发光细菌发光强度的稳定性，推进发光细菌毒性测试技术应用于在线监测和分析，以明亮发光杆菌和鳆鱼发光杆菌为对象，通过添加各种保护剂，将培养至对数生长期的菌液离心，重新悬浮于脱脂牛奶溶液冷藏（5℃），比较脱脂牛奶菌悬液冷藏、冻干粉复苏后即时冷藏以及新鲜菌液直接冷藏3种方法的调控效果.结果表明，脱脂牛奶菌悬液冷藏7d后复苏，相对发光率达到93%.该方法明显提高了发光细菌生物活性的稳定性，对于提高在线毒性监测仪连续运行时间有参考价值.图3表3参15
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