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OALib Journal期刊
ISSN: 2333-9721
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甘薯块根腐烂真菌的分离和鉴定
DOI: 10.3724/SP.J.1145.2011.00260, PP. 260-263
Keywords: 甘薯,块根,腐烂,丝状真菌,18srdna
Abstract:
从腐烂甘薯块根中分离到4株丝状真菌，分别编号为sp-1、sp-2、sp-3和sp-4，它们都能在甘薯块根片上快速生长.将这4株真菌接种在pda培养基上，观察其菌落形态和结构特征.提取4株菌的总dna，利用真菌的18srdna通用引物进行扩增、克隆和测序，其18srdna序列的长度分别为1810bp、1769bp、1768bp和1770bp.将这些序列在genbank中进行序列比对，获得的同源性较高的序列用于构建系统进化树.根据分子鉴定和形态观察的结果，sp-1菌株是匍枝根霉（rhizopusstolonifer）sp-1，sp-2是尖镰孢（fusariumoxysporum）sp-2，sp-3是康宁肉座菌（hypocreakoningii）sp-3，sp-4是肉座菌属（hypocrea），命名为hypocreasp.sp-4.图1表2参21
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