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OALib Journal期刊
ISSN: 2333-9721
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硝基苯降解菌的分离鉴定及降解特性
DOI: 10.3724/SP.J.1145.2011.00243, PP. 243-246
Keywords: 硝基苯,生物降解,假单胞菌属,基因克隆,污水处理
Abstract:
从化工厂污水处理池污泥中分离到一株能高效降解硝基苯的菌株xy-1，通过形态观察、生理生化特征和16srdna序列同源性分析，将该菌株鉴定为假单胞菌属（pseudomonassp.）.该菌株能以硝基苯为唯一碳源、氮源和能源生长，硝基苯初始浓度为200mg/l时，20h降解率可达97%.该菌在温度25~35℃、ph7.0~9.0范围内均能高效降解硝基苯，并且对对氯硝基苯、对氯苯胺也有良好的降解效果.测序分析表明，克隆到了该菌中的硝基苯还原酶基因，推测该菌的降解途径是硝基苯部分还原途径.图6参19
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