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OALib Journal期刊
ISSN: 2333-9721
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酶联免疫吸附法测定麻疯树核糖体失活蛋白
DOI: 10.3724/SP.J.1145.2011.00227, PP. 227-231
Keywords: 麻疯树,核糖体失活蛋白（curcin）,种仁,抗血清,酶联免疫吸附法
Abstract:
建立了一种检测麻疯树核糖体失活蛋白（curcin）的简便、快速的酶联免疫吸附测定法（elisa）.elisa条件优化试验结果显示：最佳条件为costar酶标板在紫外线下照射45min后进行包被；抗原最适稀释度为1:640倍（2.5μg/ml）；curcin抗血清最适稀释度为1:12800倍；2%封闭用山羊血清封闭120min；二抗最适稀释度为1:5000倍.在此试验条件下检测curcin含量，得到回归方程y=31.722x+106.88，相关系数r2=0.9933.curcin浓度在0.08~11μg/ml之间，曲线呈良好的线性关系.本方法的最低检测限为0.08μg/ml.高、中、低3个浓度的添加回收率分别为95.56%、102.27%、98.40%，平均回收率为98.74%.板内和板间的平均变异系数分别为4.78%和6.71%.curcin抗血清与2种大戟科同类蛋白均无交叉反应.利用本方法成功检测了麻疯树种仁及脱毒饼粕中的curcin含量.图3表5参19
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