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OALib Journal期刊
ISSN: 2333-9721
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外源水杨酸对梨叶片感染轮纹病菌后抗氧化酶活性及ca2+浓度的影响
DOI: 10.3724/SP.J.1145.2011.00215, PP. 215-218
Keywords: 砂梨,轮纹病菌,水杨酸,抗氧化酶,ca2+.
Abstract:
为了解水杨酸（salicylicacid，sa）缓解轮纹病菌伤害植物的机理，用0.3mmoll-1的sa预先处理梨叶片，3d后接种轮纹病菌（physalosporapiricolanose），接种4d后开始测定各种指标，随后每隔3d测定1次，共5次.结果表明，经0.3mmoll-1sa处理后，梨叶片的超氧化物歧化酶（sod）、过氧化物酶（pod）、过氧化氢酶（cat）、抗坏血酸过氧化物酶（apx）、愈创木酚过氧化物酶（gpx）和谷胱甘肽还原酶（gr）的活性分别比对照组（ck1）提高了30.65%、25.36%、28.55%、21.11%、35.67%和26.45%.电镜观察表明，感染轮纹病菌的梨叶片，ca2+较多分布在细胞膜内侧、细胞质及一些细胞器（叶绿体）上.经sa处理后感染轮纹病菌的梨病叶，ca2+较多分布在细胞壁外侧及细胞间隙中，与正常梨叶片ca2+的分布情况相似.表明较低浓度的sa可缓解轮纹病菌对梨叶片的伤害作用.图3参14
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