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OALib Journal期刊
ISSN: 2333-9721
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盐生杜氏藻烯醇酶基因启动子的克隆分析及胁迫转录应答
DOI: 10.3724/SP.J.1145.2011.00202, PP. 202-206
Keywords: 盐生杜氏藻,烯醇酶,基因组步行,启动子,实时荧光定量pcr,胁迫应答
Abstract:
为了解盐生杜氏藻（dunaliellasalina）烯醇酶（enolase）在渗透耐受中的具体功能，利用基因组步行方法和巢式pcr，从d.salina中克隆了烯醇酶基因dseno5’上游约2000bp的调控序列，并对其进行序列分析.分析表明，它包含多个与转录调控有关的保守序列（如caat-box，tata-box），富含光？干旱及其它胁迫应答元件.利用实时荧光定量pcr的方法，研究了高渗？高温以及低温外界胁迫条件下dseno的转录情况，发现其受高渗强烈抑制，高温显著诱导而低温微弱诱导.图4表2参19
| [1] | 3goyala.osmoregulationindunaliella,partⅱ:photosynthesisandstarchcontributecarbonforglycerolsynthesisduringasaltstressindunaliellatertiolecta.plantphysiolbiochem,2007,45(9):705~710
|
| [2] | 4goyala,lilleyrmcc,brownad.thesizeandturnoveroftheglycerolpoolindunaliella.plantcellenviron,1986,9(9):703~706
|
| [3] | 5brewerjm,lebiodal.currentperspectivesonthemechanismofcatalysisbytheenzymeenolase.in:bushcaed.advancesinbiophysicalchemistry.greenwich,conn:jaipress,1997,6:111~141
|
| [4] | 6iidah,yaharai.yeastheat-shockproteinofmr48,000isanisoproteinofenolase.nature,1985,315:688~690
|
| [5] | 7millerbs,kennedyte,streipsun.molecularcharacterizationofspecificheatshockproteinsinbacillussubtilis.currmicrobiol,1991,22(4):231~236
|
| [6] | 8o’sullivant,vansinderend,fitzgeraldg.structuralandfunctionalanalysisofpc165st,a6.5kbplasmidfromstreptococcusthermophilusnd1-6.microbiology,1999,145(1):127~134
|
| [7] | 13ruank,duanjb,baifw,lemairem,maxz,bailh.functionofdunaliellasalina(dunaliellaceae)enolaseanditsexpressionduringstress.eurjphycol,2009,44(2):207~214
|
| [8] | 14chitlarue,picku.selectionandcharacterizationofdunaliellasalinamutantsdefectiveinhaloadaptation.plantphysiol,1989,91(2):788~794
|
| [9] | 17sambrookj,russelld.molecularcloning:laboratorymanual.3rded.coldspringharbor,newyork,usa:coldspringharborlaboratorypress,2001
|
| [10] | 18livakkj,schmittgentd.analysisofrelativegeneexpressiondatausingreal-timequantitativepcrandthe2(-deltadeltac(t))method.methods,2001,25(4):402~408
|
| [11] | 19beckerk,pand,whitleycb.real-timequantitativepolymerasechainreactiontoassessgenetransfer.humgenether,1999,10(15):2559~2566
|
| [12] | 1avronm.theosmoticcomponentofhalotolerantalgae.trendsbiochemsci,1986,11(1):5~6
|
| [13] | 2ben-amotza,avronm.theroleofglycerolintheosmoticregulationofthehalophilicalgadunaliellaparva.plantphysiol,1973,51(5):875~878
|
| [14] | 9lalsk,johnsons,conwayt,kelleypm.characterizationofamaizecdnathatcomplementsanenolase-deficientmutantofescherichiacoli.plantmolbiol,1991,16(5):787~795
|
| [15] | 10leeh,guoy,ohtam,xionglm,stevensonb,zhujk.los2,ageneticlocusrequiredforcold-responsivegenetranscriptionencodesabi-functionalenolase.emboj,2002,21(11):2692~2702
|
| [16] | 11pandeyak,jainp,podilagk,tudzynskib,davismr.coldinducedbotrytiscinereaenolase(bcenol-1)functionsasatranscriptionalregulatorandiscontrolledbycamp.molgenetgenomics,2009,281(2):135~146
|
| [17] | 12bailh(白林含),qiaodr(乔代蓉),zhengm(郑鸣),xuh(徐辉),liq(李茜),jiangy(蒋彦),yangzh(杨志荣),caoy(曹毅).cloningenolasegeneofdunaliellasalinawithmodifieddegeneratepcrtechnology.jbeijingforuniv(北京林业大学学报),2004,26(4):25~29
|
| [18] | 15siebertpd,chenchika,kelloggde,lukyanovka,lukyanovsa.animprovedpcrmethodforwalkinginunclonedgenomicdna.nucleicacidsres,1995,23(6):1087~1088
|
| [19] | 16noguchia,takekawan,einarsdottirt,kouram,noguchiy,takanok,yamamotoy,matsudaj,suzukio.chromosomalmappingandzygositycheckoftransgenesbasedonflankinggenomesequencesdeterminedbygenomicwalking.expanim,2004,53(2):103~111
|
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