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OALib Journal期刊
ISSN: 2333-9721
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水稻ddb1基因的表达特性及功能分析
DOI: 10.3724/SP.J.1145.2011.00001, PP. 1-6
Keywords: ddb1基因,rna干扰,果实特异启动子,农杆菌介导转化,雄性半不育,水稻
Abstract:
利用rna干涉技术构建水稻ddb1（damageddnabindingprotein1）基因不同启动子驱动植物表达载体，ddb1-rnai和ddb1-glu-rnai.通过根癌农杆菌介导转入水稻愈伤，经组织培养成功获得转基因植株.半定量rt-pcr分析显示，与野生型植株相比，两组转基因植株幼叶内ddb1的表达量均有不同程度的降低.花粉染色结果显示，转基因植株花粉染色异常，败育率高；植株表现出明显的雄性半不育；秕谷率均有不同程度的提高；并且以上表型在ddb1-rnai转基因植株中均比在ddb1-glu-rnai转基因植株中明显.因此，推断ddb1基因与水稻的生殖发育有着密切的关系.图6表1参30
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