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OALib Journal期刊
ISSN: 2333-9721
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天祝高寒草地三线镰孢的分离鉴定及β-tubulin基因序列分析
DOI: 10.3724/SP.J.1145.2011.00051, PP. 51-56
Keywords: 天祝高寒草地,三线镰孢,鉴定,β-tubulin基因
Abstract:
按30m的梯度将海拔2880~3360m分为16个采样点，在同一海拔高度按照“z”字形取样，共取5个点，对16个采样点的土样混匀后以根残体分离法在镰刀菌选择性培养基上进行分离，对分离得到的镰刀菌菌落进行纯化和单孢分离后，以形态学为基础，参照nelson分类系统进行鉴定.结果表明：在分离到的212个镰刀菌菌株中，有5个菌株为三线镰孢（fusariumtricinctum）.随机选取2株三线镰孢菌株进行β-tubulin基因序列分析，将pcr产物回收测序后在genbank上比对，菌株tzh-4-3-7和tzh-11-5-2与genbank上登记的6个新西兰菌株的亲缘关系最近，同源性达96%.利用dnastar软件绘制其系统发育树状图，结果显示，菌株tzh-4-3-7和tzh-11-5-2与以上6个f.tricinctum菌株均位于系统发育树的同一分支，聚为一类.本研究采用的β-tubulin基因序列分析结果验证了利用形态学鉴定镰刀菌的准确性；将β-tubulin基因序列分析应用于三线镰孢的鉴定中，拓宽了鉴定途径，提高了鉴定的准确性；并了解了三线镰孢在天祝高寒草原不同海拔高度的分布，丰富了我国草原镰刀菌资源库，为进一步探讨镰刀菌与草原退化的关系奠定了基础.图4参45
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