|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
长柄石杉居群遗传多样性和遗传结构的aflp分析
DOI: 10.3724/SP.J.1145.2011.00018, PP. 18-23
Keywords: 长柄石杉,aflp,遗传多样性,居群,遗传结构,武夷山
Abstract:
石杉科植物因所含石杉碱甲（huperzinea）对中老年痴呆等具有良好疗效，近年来倍受关注.利用aflp分子标记对武夷山脉广布种长柄石杉[huperziaserrata(thunb.exmurray)trev.var.longipetiolata(spring)h.m.chang]7个居群112株个体进行遗传多样性和居群遗传结构分析.选用多态性高、分辨力强的8对选择性扩增引物组合共获得675个位点，其中多态位点比例为69.38%.居群内观测等位基因数（na）为1.633，有效等位基因数（ne）1.493；nei’s基因多样性指数（he）与shannon多态性信息指数（i）的平均值分别为0.272和0.392，多样性最高为地处武夷山脉中段的泰宁居群和建宁居群，最低为山脉北段的光泽居群.居群总基因多样性（ht）为0.3273，居群内基因多样性（hs）为0.2722，居群间的遗传分化系数（gst）为0.1681，表明居群内变异是长柄石杉遗传多样性的主要来源.由gst估计，武夷山脉长柄石杉自然居群的基因流（nm）为2.4740.邻接树分析表明居群间遗传亲缘关系与地理位置相关.武夷山脉长柄石杉较高的遗传多样性和基因流水平表明其仍然具有相当的适应（生存）能力和进化潜力，这可能与其生物学（异交水平）、生态学特性及武夷山脉相对良好的生境条件有关.图2表5参41
| [1] | 1wangye(王月娥),yuedx(岳东贤),tangxc(唐希灿).anti-cholinesteraseactivityofhuperzinea.actapharmacolsin(中国药理学报),1986,7(2):110
|
| [2] | 2maxq,gangdr.thelycopodiumalkaloids.natprodrep,2004,21(6):752~772
|
| [3] | 3maxq,tanch,zhudy,gangdr.isthereabettersourceofhuperzineathanhuperziaserrata?huperzineacontentofhuperziaceaespeciesinchina.jagric&foodchem,2005,53:1393~1398
|
| [4] | 4maxq,tanch,zhud,xiaop.huperzineafromhuperziaspecies-anethnopharmacologicalreview.jethnopharmacol,2007,113(1):15~34
|
| [5] | 5wangj(王峻),pansl(潘胜利).studyoncontentofhuperzineainsixplantsofhuperziafromhunanprovince.chinpharmj(中国药学杂志),2005,40(21):1616~1618
|
| [6] | 6jisg(姬生国),pansl(潘胜利),wangj(王峻).phylogenyrelationshipandmolecularidentificationoftenhuperziaspecies(huperziaceae)basedonmatkgenesequeuces.chinajchinmatmed(中国中药杂志),2007,32(19):1971~1975
|
| [7] | 7jisg,huokk,wangj,pansl.amolecularphylogeneticstudyofhuperziaceaebasedonchloroplastrbclandpsba-trnhsequences.jsyst&evol,2008,46(2):213~219
|
| [8] | 12doylejj,doylejl.arapiddnaisolationprocedureforsmallquantitiesoffreshleafmaterial.phytochembull,1987,19:11~15
|
| [9] | 13yehfc,yangr,boyletj.popgene32,microsoftwindows-basedfreewareforpopulationgeneticanalysis(version1.32).alberta,canada:molecularbiologyandbiotechnologycentre,universityofalberta,2000
|
| [10] | 14peakallr,smousep.genalex6:geneticanalysisinexcel.populationgeneticsoftwareforteachingandresearch.molecolnotes,2006,6:288~295
|
| [11] | 16zhivotovskyla.estimatingpopulationstructureindiploidswithmultilocusdnamarkers.molecol,1999,8:907~913
|
| [12] | 17felsensteinj.phylip(phylogenyinferencepackage,version3.6).distributedbytheauthor.seattle,usa:departmentofgenomesciences,universityofwashington,2004
|
| [13] | 18yew(叶文),lizj(李振基),benitoctan,chenlz(陈鹭真),hejy(何建源),liucd(刘初钿).studyonthebiodiversityoffernsfromsoutherntonorthernwuyimoutains.jxiamenuniv(厦门大学学报自然科学版),2007,46(3):431~437
|
| [14] | 19busogsc,rangelph,ferreirame.analysisofgeneticvariabilityofsouthamericanwildricepopulations(oryzaglumaepatula)withisozymesandrapdmarkers.molecol,1998,7:107~117
|
| [15] | 20ennosra.estimatingtherelativeratesofpollenandseedmigrationamongplantpopulations.heredity,1994,72:250~259
|
| [16] | 27zhouhg(周厚高),xieyl(谢义林),lih(黎桦),zhouq(周琼),zhangxl(张西丽),wangzr(王中仁),zhousl(周世良).analysisofgeneticdiversityonpterisvittatal.populationsfromguangxilimestonearea.guihaia(广西植物),2002,22(1):67~71
|
| [17] | 28wangkq(王可青),wangzr(王中仁).theallozymicvariationindiploidaspleniumsareliihook.(aspleniaceae).actagensin(遗传学报),1998,25(5):454~463
|
| [18] | 29panglq(潘丽芹),jih(季华),chenlq(陈龙清).geneticdiversityofthenaturalpopulationsofadiantumreniformevar.sinense.biodivsci(生物多样性),2005,13(2):122~129
|
| [19] | 30nybomh.comparisonofdifferentnucleardnamarkersforestimatingintraspecificgeneticdiversityinplants.molecol,2004,13:1143~1155
|
| [20] | 31madw(马丹炜),wangsh(王胜华),luot(罗通),wangwg(王文国),zhuanggq(庄国庆),chenf(陈放).comparitivestudyongeneticdiversityofrockplantpogonatherumpaniceumusingissrandaflpmarkers.chinjapplenvironbiol(应用与环境生物学报),2006,12(5):605~608
|
| [21] | 32chenjm,wangjy,liux,gituruwr,wangqf.rapdanalysisforgeneticvariationwithintheendangeredquillwortisoeteshypsophila(isoetaceae).wuhanunivjnatsci,2005,10(2):455~459
|
| [22] | 35hamrickjl,godtmjw.allozymediversityinplantspecies.in:brownadh,cleggmt,kahleraleds.plantpopulationgenetics,breedingandgeneticresources.sunderland,usa:sinauerassocinc,1989.43~63
|
| [23] | 36gexj,zhanglb,yuanym,haog,chiangty.stronggeneticdifferentiationoftheeast-himalayanmegacodonstylophorus(gentianaceae)detectedbyinter-simplesequencerepeats(issr).biodiv&conserv,2005,14:849~861
|
| [24] | 39busselljd.thedistributionofrandomamplifiedpolymorphicdna(rapd)diversityamongstpopulationsofisotomapetraea(lobeiaceae).molecol,1999,8:775~789
|
| [25] | 40hogbinpm,peakallr.evaluationofthecontributionofgeneticresearchtothemanagementoftheendangeredplantzieriaprostrate.conservbiol,1999,13:514~522
|
| [26] | 8tangsq,binxy,zhongy.assessmentofgeneticdiversityincultivarsandwildaccessionsofluohanguo(siraitiagrosvenorii[swingle]a.m.luetz.y.zhang),aspecieswithedibleandmedicinalsweetfruitsendemictosouthernchina,usingrapdandaflpmarkers.genres&cropevol,2007,54:1053~1061
|
| [27] | 9lizj(李振基),linp(林鹏),yew(叶文),chenlz(陈鹭真),qiul(裘丽),chensb(陈圣宾),liucd(刘初钿),hejy(何建源),daids(戴德升),lil(李林).thebiodiversityflowoffernsfromsoutherntonorthernwuyimoutains.prognatsci(自然科学进展),2006,16(8):959~964
|
| [28] | 10wangj(王峻),pansl(潘胜利),shunqs(顺庆生),wanggy(王光远).aweapontoseniledementia-hupaandthemedicinalplantsofhuperziaceaeinchina.shanghai,china:shanghaiscientifc&technologicalliteratrepublishinghouse(上海:上海科技文献出版社),2008.25
|
| [29] | 11zhangjc(张君诚),songyh(宋育红),huangh(黄晖),zhanghy(张杭颖),chenjm(陈建民).investigationonthefeatureofhuperziaceaecommunityatsanmingareainfujianprovince.jfudanuniv(复旦学报自然科学版),2008,47(5):627~632
|
| [30] | 15vekemansx.aflp-surv(version1.0).distributedbytheauthor.brussels,belgium:laboratoiredegénétiqueetecologievégétale,universitélibredebruxelles,2002
|
| [31] | 21yanj(闫娟),chuhj(楚海家),wanghc(王恒昌),lijq(李建强).geneticstructureanddiversityofmedicagolupulinapopulationsinnorthernandcentralchinabasedonest-ssrsmarkers.biodivsci(生物多样性),2008,16(3):263~270
|
| [32] | 22govindarajudr.relationshipbetweendispersalabilityandlevelsofgeneflowinplants.oikos,1988,52:31~35
|
| [33] | 23lovelessmd,hamrickjl.ecologicaldeterminantsofgeneticstructureinplantpopulations.annrevecolsyst,1984,15:65~95
|
| [34] | 24chenyy(陈媛媛),yeqg(叶其刚),lizz(李作洲),huanghw(黄宏文).geneticstructureofxiuningpopulationofisoetessinensis,acriticallyendangeredspeciesinchina.biodivsci(生物多样性),2004,12(6):564~571
|
| [35] | 25chenjm(陈进明),wangjy(王晶苑),liux(刘星),zhangyw(张彦文),wangqf(王青锋).rapdanalysisforgeneticdiversityofisoetessinensis.biodivsci(生物多样性),2004,12(3):348~353
|
| [36] | 26chenjm(陈进明),liux(刘星),wangqf(王青锋).geneticdiversityinisoetesyunguiensis,arareandendangeredendemicferninchina.wuhanunivjnatsci(武汉大学学报理学版),2005,51(6):767~770
|
| [37] | 33hsutw,mooresj,chiangty.lowrapdpolymorphisminarchangiopterisitoi,arareandendemicfernintaiwan.botbullacadsin,2000,41(1):15~18
|
| [38] | 34ges(葛颂),hongdy(洪德元).studiesofmorphologicalandallozymevariationoftheendangeredadenophoralobophyllaanditswidespreadcongenera.potaninii.actagensin(遗传学报),1999,26(4):410~417
|
| [39] | 37whittierdp.gametophytesoffourtropical,terrestrialhuperziaspecies(lycopodiaceae).amfernj,2006,96:54~61
|
| [40] | 38wangxl(王小理),liwk(王可青),zhoujy(周吉燕).morphologicalstudyongemmaeandgemmalingsofhuperziajavanica(huperziaceae).actabotyunnanica(云南植物研究),2007,29(5):521~526
|
| [41] | 41chenghy(程宏毅),baoyx(鲍毅新),chenl(陈良),huzy(胡知渊),gebm(葛宝明).effectsofhabitatfragmentationongeneflowoftheblackmuntjac(muntiacuscrinifrons).actaecolsin(生态学报),2008,28(3):1109~1118
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
