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OALib Journal期刊
ISSN: 2333-9721
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水稻osgpcr基因的克隆及遗传转化分析
DOI: 10.3724/SP.J.1145.2011.00007, PP. 7-11
Keywords: 水稻g蛋白偶联受体,7次跨膜结构域,农杆菌介导转化,赤霉素,转基因水稻
Abstract:
g蛋白偶联受体（gprotein-coupledreceptors，gpcr）基因家族是目前已知最大的膜受体超家族，参与生物的生长发育调控和激素应答.通过rt-pcr分离了水稻中一个具有7次跨膜（seventransmembrane，7tm）结构域的osgpcr候选基因；分析该基因启动子序列，发现它含有赤霉素ga应答相关元件.为分析其功能，分别构建osgpcr过表达和rna干涉载体，并通过农杆菌介导法转化获得转基因水稻植株.实验结果表明，较之野生型，过表达转基因植株对赤霉素反应更敏感，rna干涉植株则反之.图7表2参19
| [1] | 5gutkindjs.thepathwaysconnectinggprotein-coupledreceptorstothenucleusthroughdivergentmitogen-activatedproteinkinasecascades.jbiolchem,1998,273(4):1839~1842
|
| [2] | 6dymocksp,dymockd,hooleyr.ahigherplantseven-transmembranereceptorthatinfluencessensitivitytocytokinins.currbiol,1998,8:315~324
|
| [3] | 11xuey(薛颖),huangwz(黄维藻),zhuot(卓婷),tangzz(唐子执),ninxl(牛向丽),liuys(刘永胜).isolationofricerbx1cdnaanditsgenetictransformationofrbx1over-expressionvector.chinjapplenvironbiol(应用与环境生物学报),2008,14(3):337~340
|
| [4] | 12floreal,hartzellg,zhangz,rubingm.millerw.acomputerprogramforaligningacdnasequencewithagenomicdnasequence.genomeres,1998,8:967~974
|
| [5] | 15zhangzl,xiez,zoux,casarettoj,davidth,shenqj.aricewrkygeneencodesatranscriptionalrepressorofthegibberellinsignalingpathwayinaleuronecells.plantphysiol,2004,134:1500~1513
|
| [6] | 1jacobye,bouhelalr,gerspacherm,seuwenk.the7tmg-protein-coupledreceptortargetfamily.chemmedchem,2006,1(8):760~782
|
| [7] | 2oldhamwm,hammhe.heterotrimericgproteinactivationbyg-protein-coupledreceptors.nature,2008,9:60~71
|
| [8] | 3getheru.uncoveringmolecularmechanismsinvolvedinactivationofgprotein-coupledreceptors.endocrinerev,2000,21(1):90~113
|
| [9] | 4deleana,stadeljm,lefkowitzrj.aternarycomplexmodelexplainstheagonist-specificbindingpropertiesoftheadenylatecyclase-coupledb-adrenergicreceptor.jbiolchem,1980,255:7108~7117
|
| [10] | 7pandeys,assmannsm.thearabidopsisputativegprotein-coupledreceptorgcr1interactswiththegproteinαsubunitgpa1andregulatesabscisicacidsignaling.plantcell,2004,16:1616~1632
|
| [11] | 8tanakamu,fujisaway,kobayashim,ashikarim,iwasakiy,kitanoh,matsuokam.ricedwarfmutantd1,whichisdefectiveintheasubunitoftheheterotrimericgprotein,affectsgibberellinsignaltransduction.procnatlacadsci,2000,97(21):11638~11643
|
| [12] | 9okik,inaban,kitagawak,fujiokas,kitanoh,fujisaway,katoh,iwasakiy.functionoftheasubunitofriceheterotrimericgproteininbrassinosteroidsignaling.plantcellphysiol,2009,50(1):161~172
|
| [13] | 10gaoyf(高永峰),liujk(刘继恺),fanj(范晶),pengy(彭奕),huangk(黄科),zhangdf(张敦房),liuys(刘永胜).constructionandtransformationofrnaivectorofosvp1foraregulatorygeneofpre-harvestsproutinginoryzasativa.sciagricsin(中国农业科学),2010,43(7):1321~1327
|
| [14] | 13higok,ugaway,iwamotom,korenagat.plantcis-actingregulatorydnaelements(place)database:1999.nuclacidsres,1999,27(1):297~300
|
| [15] | 14changlj(常丽娟),niuxl(牛向丽),luod(罗笛),zhouyk(周宇科),luoxl(罗晓莉),xiel(谢亮),liuq(刘茜),zhoum(周觅),liuys(刘永胜).expressioncharacteristicsofextracellularnucleosidetriphosphate-diphosphohydrolasesgeneinoryzasativa.chinjapplenvironbiol(应用与环境生物学报),2009,15(6):768~773
|
| [16] | 16gookinte,kimj,assmannsm.wholeproteomeidentificationofplantcandidateg-proteincoupledreceptorsinarabidopsis,rice,andpoplar:computationalpredictionandin-vivoproteincoupling.genomebiol,2008,9:r120
|
| [17] | 17moriyamaen,stropepk,opiyoso,chenz.miningthearabidopsisthalianagenomeforhighlydivergentseventransmembranereceptors.genomebiol,2006,7(10):r96
|
| [18] | 18fujisaway,katot,ohkis,ishikawaa,kitanoh,sasakit,asahit,iwasakiy.suppressionoftheheterotrimericgproteincausesabnormalmorphology,includingdwarfism,inrice.procnatlacadsci,1999,96:7575~7580
|
| [19] | 19assmannsm.gproteinsignalingintheregulationofriceseedgermination.science,2005,310:12
|
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