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OALib Journal期刊
ISSN: 2333-9721
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响应面法优化锰氧化细菌发酵培养基
DOI: 10.3724/SP.J.1145.2011.00130, PP. 130-134
Keywords: 锰氧化细菌,生物除锰,培养基优化,响应面法,plackett-burman设计
Abstract:
bacillussp.wh4是一株锰氧化能力强的锰氧化细菌，在锰污染的水源净化方面具有较好的应用前景.为提高发酵液中bacillussp.wh4的活菌数，应用响应面法优化其发酵培养基.plackett-burman设计获得对活菌数有显著影响的4个因素：豆粕粉、kh2po4、mgso4、feso4；最陡爬坡试验确定中心组合实验的最大响应区域；中心组合设计和响应面分析优化出4个显著因素的最佳浓度.优化后的最适培养基（w/%）组成为：蔗糖2、豆粕粉0.96、k2hpo40.8、kh2po40.19、mgso4？7h2o0.11、cacl20.02、nacl0.5、feso4？7h2o0.02.发酵试验表明，最优培养基的活菌数可达4.2×109cfuml-1，比初始发酵培养基提高近3倍.图1表6参15
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