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OALib Journal期刊
ISSN: 2333-9721
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反应条件对苛求芽孢杆菌胞内尿酸酶与嘌呤衍生物作用的影响
DOI: 10.3724/SP.J.1145.2011.00091, PP. 91-94
Keywords: 尿酸酶,嘌呤衍生物,尿酸,苛求芽孢杆菌,活化能,亲和力
Abstract:
用底物尿酸的紫外吸收变化跟踪反应，用单或双底物米氏方程和初速度估算苛求芽孢杆菌胞内尿酸酶最大反应速度，按arrenius经验公式得到该尿酸酶在ph9.2和ph7.4的活化能，但这些活化不能解释其在两个ph下的催化活性差异.该酶同常见嘌呤衍生物氧嗪酸、黄嘌呤和尿酸有亲和力，但对尿酸亲和力最低，且亲和力与嘌呤衍生物解离成阴离子的能力相关.在生理条件下，该酶对尿酸的亲和力相对于最适条件有显著下降.据此推测，优化静电相互作用选择性增强该酶对尿酸的亲和力是提高其成药性的可能措施之一.图2表4参29
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