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OALib Journal期刊
ISSN: 2333-9721
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利用细菌表面展示技术构建镉耐受性的重组根瘤菌
DOI: 10.3724/SP.J.1145.2011.00082, PP. 82-86
Keywords: 细菌表面展示,金属硫蛋白,重组根瘤菌,镉吸附,镉耐受
Abstract:
通过细菌表面展示功能基因inaxn、猴金属硫蛋白α亚基四聚体mt4α和两种启动子pnifh及plac元件，分别构建两个重组质粒ptnim和pblim.进一步通过三亲本杂交，将重组质粒分别导入费氏中华根瘤菌hn01，分别构建获得诱导表达型重组菌hn01（ptnim）和组成表达型重组菌hn01（pblim）.在培养条件下比较测定重组菌和出发菌对镉的吸附能力和耐受性，结果表明，组成表达型重组菌hn01（pblim）在上述两个方面的能力得到明显增强，对镉的吸附富集能力提高了2倍.研究还发现重组根瘤菌也具有很好的静态吸附效果.盆栽实验结果表明，接种诱导表达型重组菌hn01（ptnim）的大豆根瘤和根中cd2+的富集量均明显高于野生菌株hn01.本实验为后续探索重组根瘤菌与宿主植物共生体系在cd2+污染土壤修复中的应用前景奠定了基础.图5表1参22
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