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OALib Journal期刊
ISSN: 2333-9721
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非生物胁迫对盐生杜氏藻dsmpk转录、翻译和磷酸化修饰的影响
DOI: 10.3724/SP.J.1145.2011.00029, PP. 29-33
Keywords: 盐生杜氏藻,促有丝分裂活化蛋白激酶,非生物胁迫,信号调控,磷酸化修饰
Abstract:
促有丝分裂活化蛋白激酶（mitogen-activatedproteinkinase，mapk或mpk）途径是在真核生物中广泛存在的调控多种生理过程的信号途径，在细胞增殖、分化、凋亡、生物和非生物胁迫等生理过程的调控中起着关键的作用.盐生杜氏藻（dunaliellasalina，盐藻）是目前世界上最耐盐的真核光合生物之一.在前期实验中，已发现盐藻mapk（dsmpk）的表达受到低温和高盐胁迫的抑制.分析dsmpk在高温、低盐、氧化、紫外胁迫中的表达变化，发现dsmpk还受到氧化胁迫的抑制.进而分析dsmpk在高温、低盐、氧化、低温、高盐和紫外胁迫中翻译和磷酸化修饰的变化，发现除低盐胁迫外，在磷酸化修饰水平dsmpk同样受到各种非生物胁迫的抑制.在对不同生长阶段盐藻dsmpk磷酸化变化的分析中还发现，dsmpk的磷酸化水平与生长速率有一致性.各方面dsmpk的变化趋势说明其为一调控生长相关的mapk.图4参29
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