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OALib Journal期刊
ISSN: 2333-9721
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石油烃降解酶及其基因的结构、功能和表达调控
DOI: 10.3724/SP.J.1145.2012.01066, PP. 1066-1074
Keywords: 石油微生物,烃,加氧酶,苯酰琥珀酸合酶,乙苯脱氢酶,基因表达调控
Abstract:
研究烃降解酶及其基因是进行石油微生物分子检测和工程菌构建的重要基础.本文对目前烃降解酶及其基因的结构、功能和调控机制的最新研究进展进行了总结.催化烷烃好氧降解的起始酶有几类加氧酶，膜整合甲烷单加氧酶、萘-1,2-双加氧酶和异丙苯双加氧酶的晶体结构已经被解析.烷基或芳基琥珀酸合酶催化烃厌氧代谢的主要起始反应，而azoarcussp.乙苯厌氧代谢起始反应由乙苯脱氢酶催化.在细菌中，烃代谢相关基因主要通过形成操纵子进行表达调控，基因转录受烃或类似物诱导，并受细胞全局调控.一些微生物由于存在多种烃代谢途径而可能具有复杂的基因调控机制.此外，生态学研究表明，环境中烃降解基因的诱导动态与实验室内纯培养分析不同.在分析石油降解工程菌构建解决问题的基础上，提出了烃代谢综合调控和环境中相关酶及基因诱导研究的重要性，并对未来烃降解酶及其基因在有毒物降解理论研究和生物修复上的应用进行了展望.图3参50
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