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OALib Journal期刊
ISSN: 2333-9721
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一株积聚四氢嘧啶的青海湖盐单胞菌的分离及特性
DOI: 10.3724/SP.J.1145.2012.01049, PP. 1049-1055
Keywords: 青海湖,盐单胞菌,分离鉴定,四氢嘧啶
Abstract:
从青海湖采集20份水样，利用rm高盐培养基分离获得35株嗜盐菌.选取其中一株优势中度嗜盐菌qhl25进行形态学和生理生化特征分析，结果表明：qhl25菌落圆形或椭圆形，乳白色，不透明，中心突出，边缘透明，生长盐度为0~2.9mol/l，最适生长盐度为1.45mol/l.显微形态呈短杆状，革兰氏染色呈阴性，单个或成串排列.最适生长ph为8.5，最适生长温度为37℃.16srdna序列分析表明：该菌株与盐单胞菌属（halomonassp.）相似性为98%；结合形态特征和生理生化特征分析，初步认定该菌株隶属于γ变形杆菌纲（gammaproteobacteria）海洋螺菌目（oceanospirillales）盐单胞菌科（holomonadaceae）盐单胞菌属（holomonas）.薄层层析（tlc）定性分析可知qhl25胞内含有相容溶质四氢嘧啶；高效液相色谱（hplc）定量分析表明，在1.7mol/l的nacl浓度下，四氢嘧啶含量约为36.70mg/g（细胞干重）.图6表2参30
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