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OALib Journal期刊
ISSN: 2333-9721
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多管发酵-菌落pcr法定量检测水中可培养沙门氏菌
DOI: 10.3724/SP.J.1145.2012.01004, PP. 1004-1008
Keywords: 可培养沙门氏菌,多管发酵-菌落pcr法,膜吸附-洗脱法,定量检测,最大可能数法
Abstract:
采用膜吸附-洗脱法浓缩水样，通过多管发酵法筛选出以可培养沙门氏菌为主的活体细菌.根据沙门氏菌inva基因的保守性，利用沙门氏菌属通用引物进行菌落pcr鉴定，进而确定沙门氏菌阳性平板数量.运用最大可能数法分析数据，计算出水样中可培养沙门氏菌的含量.该方法对沙门氏菌具有良好的特异性和涵盖性.通过对人工污染水样和城市污水厂二级处理出水的检测，证明了多管发酵-菌落pcr方法的检测结果较为准确，可操作性强，能够更真实地反映样品中沙门氏菌的活性.图3表2参13
| [1] | 1worldhealthorganization.guidelinesforsaferecreationalwaterenvironments(volume1:coastalandfreshwaters).geneva,switzerland:worldhealthorganization,2003
|
| [2] | 2bellc,kyriakidesa.salmonella.apracticalapproachtotheorganismanditscontrolinfoods.practicalfoodmicrobiologyseries.oxford,unitedkingdom:blackwellscienceltd.,2002
|
| [3] | 4baudartj,lemarchandk,brisaboisa,lebaronp.diversityofsalmonellastrainsisolatedfromtheaquaticenvironmentasdeterminedbyserotypingandamplificationoftheribosomaldnaspacerregions.applenvironmicrobiol,2000,66:1544~1552
|
| [4] | 5sunyy(孙园园),zhaop(赵鹏),liuj(刘骏),wangfh(王富海),qiank(钱科),zhujl(朱金连).researchprogressofsalmonelladetectionmethods.chinaanimhusbvetmed(中国畜牧兽医),2011,38(1):218~220
|
| [5] | 7kwonhj,parkky,yoohs,parkjy,parkyh,kimsj.differentiationofsalmonellaentericaserotypegallinarumbiotypepullorumfrombiotypegallinartbyanalysisofphase1flagellincgene(flic).jmicrobiolmethods,2000,40(1):33~38
|
| [6] | 8hashimotoy,ithoy,fujinagay,khanaq,sultanaf,miyakem,hirosek,yamamotoh,ezakit.developmentofnestedpcrbasedontheviabsequencetodetectsalmonellatyphi.jclinmicrobiol,1995,33(3):775~777
|
| [7] | 10malornyb,hoorfarj,bungec,helmuthr.multicentervalidationoftheanalyticalaccuracyofsalmonellapcr:towardsaninternationalstandard.applenvironmicrobiol,2003,69(1):290~296
|
| [8] | 12shens(沈圣),jianghz(江华洲),qiuj(邱晋),xux(许欣),peixf(裴晓方).confirmationofdetectionmethodofsalmonellabyapplyingstudymethodologyofevidence-basedmedicine(ebm).jprevmedinf(预防医学情报杂志),2006,22(1):106~109
|
| [9] | 13weimn(魏梦楠),zhangcm(张崇淼),wangxc(王晓昌),zengs(曾颂).improvedmpnquantitativedetectionmethodofsalmonellaintreatedwastewater.jenvironhealth(环境与健康杂志),2010,27(1):59~61
|
| [10] | 3espigarese,buenoa,espigaresm,gálvezr.isolationofsalmonellaserotypesinwastewaterandeffluent:effectoftreatmentandpotentialrisk.intjhygenviron-health,2006,209:103~107
|
| [11] | 6parksh,kimhj,chowh,kimjh,ohmh,kimsh,leebk,rickesc,kimhy.identificationofsalmonellaentericasubspeciesi,salmonellaentericaserovarstyphimurium,enteritidisandeyphiusingmultiplexpcr.femsmicrobiollett,2009,301:137~146
|
| [12] | 9rahna,degrandissa,clarkerc,mcewensa,galánje,ginocchioc,curtissiiir,gylescl.amplificationofaninvagenesequenceofsalmonellatyphimuriumbypolymerasechainreactionasaspecificmethodofdetectionofsalmonella.molecellprobe,1992,6:271~279
|
| [13] | 11malornyb,hoorfarj,hugasm,heuvelinka,fachp,ellerbroekl,bungec,dornc,helmuthr.interlaboratorydiagnosticaccuracyofasalmonellaspecificpcr-basedmethod.intjfoodmicrobiol,2003,89(2~3):241~249
|
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