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OALib Journal期刊
ISSN: 2333-9721
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一株高产油脂丝状真菌的形态学及rdna-its2分子鉴定
DOI: 10.3724/SP.J.1145.2012.00988, PP. 988-992
Keywords: 易脆毛霉,形态鉴定,rdna-its2,微生物油脂
Abstract:
从采集的土样中分离获得45株产油脂菌株，其中丝状真菌7-4的原始油脂含量最高，达到46.80%.分别采用传统形态学鉴定方法及rdna-its2序列克隆进行鉴定.形态学鉴定的结果初步表明该真菌为卷枝毛霉（mucorcircinelloides），采用rdna-its2序列进行复核表明该菌与卷枝毛霉（m.circinelloides）同源性为98.00%，而与易脆毛霉（m.fragilis）同源性达到100.00%.从构建的系统进化树中可以看出，该菌与易脆毛霉共同构成一个分支，并与卷枝毛霉聚成一大枝.结合形态学鉴定的结果确定菌株7-4为易脆毛霉（m.fragilis）.其脂肪酸组成与植物相似，以棕榈酸、亚油酸、油酸为主，不饱和脂肪酸含量达91.77%.图5表3参16
| [1] | xuefy,miaojx,zhangx.studiesonlipidproductionbyrhodotorulaglutinisfermentationusingmonosodiumglutamatewastewaterasculturemedium.bioresourtechnol,2008,99:5923
|
| [2] | zhuds(朱丹实),liuh(刘贺),lüyf(吕艳芳).researchprogresonoil-producingfungus.cerealsoilsprocess(油脂工程?技术),2009,6:54~57
|
| [3] | zhenghb(郑洪波),liugh(刘光华),lixr(李欣然),lij(李�),xiej(谢洁),qiaodr(乔代蓉),caoy(曹毅).screeningandidentificationoffat-producingmicroorganismsandanalysisofitsfattyacidcomposition.jsichuanunivnatscied(四川大学自然科学版)2010,47(6):1397~1401
|
| [4] | wangww(王卫卫),renpk(任鹏康).selectivebreedingofgammalinolenicacidhigh-yieldingstrainofrhizopusarrhizusandstudiesonsolidstatefermentationconditions.mycosystema(菌物系统),2002,21(1):92~97
|
| [5] | longlk(龙良鲲),yaoq(姚青),yangsz(羊宋贞),zhuhh(朱红惠).identificationofanarbuscularmycorrhizalfungibythemethodsofmorphologyandmolecule.jsouthchinaagricuniv(华南农业大学学报),2006,27(4):40~42
|
| [6] | whitetj,brunst,lees.analysisofphylogeneticrelationshipsbyamplificationanddirectsequencingofribosomalrnagenes.newyorkacademic,1990.15~22
|
| [7] | baism(白树猛),tianl(田黎).applicationofitssequencesinclassificationandidentificationandmoleculartestoffungi.animhusbandryfeedsci(畜牧与饲料科学),2009,30(1):52~53
|
| [8] | zhaozb(赵宗保).towardcheapermicrobialoilforbiodieseloil.chinbiotechnol(生物工程),2005,25(2):8~11
|
| [9] | malj(马丽娟),wanghm(王海明),wenyf(温玉凤),jinghl(姜会丽).optimizedculturemediumandfermentationconditionsforlipidproductionwithfilamentousfungus.jchangchununivtechnol(长春理工大学学报),2008,29(4):459~463
|
| [10] | yaos(姚粟),lih(李辉),chengc(程池).studiesonidentificationof23aspergillussp.strainsinmorphology.food&fermentind(食品与发酵工业),2006,32(12):37~43
|
| [11] | deepakp,aloka.identificationligninolyticenzymeactivityanddecolorizationpotentialoftwofungiisolatedfromadistilleryeffluentcontaminatedsite.waterair&soilpollution,2007,183(14):165~176
|
| [12] | lauraev,josephof.comparisonofmethodsforisolationofamycobacteriumaviumcomplexdnaforuseinpcrandrapdfingerprinting.jmcirobiolmethods,1995,21(2):151~161
|
| [13] | wangy(王英),guj(顾军),liuwd(刘维达).rapiddetectionandidentificationofcandidaspecieswithitsuniversalprimersbypcr.jclindermatol(临川皮肤科杂志),2003,32(2):69~71
|
| [14] | turennecy,sanchese,hobandj.rapididentificationoffungibyusingtheits2geneticregionandanautomatedfluorescentcapillaryelectrophoresissystem.jclinmicrobiol,1990,37(6),1846~1851
|
| [15] | ratledgec.awonderlandforthemicrobialphysiologist.jamoilchemsoc,1987,64:1647~1656
|
| [16] | heyt(何毅婷),yangyd(杨汝德),zhangg(张广),huangyw(黄耀威).identificationofacellulose-producingfungibasedon18srdnasequence.modfoodscitechnol(现代食品科技),2008,24(7):638~639
|
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