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OALib Journal期刊
ISSN: 2333-9721
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4种重金属离子对克氏原螯虾（procambarusclarkii）nagase活力的影响
DOI: 10.3724/SP.J.1145.2012.00943, PP. 943-947
Keywords: 克氏原螯虾,n-乙酰-β-d-氨基葡萄糖苷酶,酶活力,金属离子,作用机理
Abstract:
研究了ag+、pb2+、zn2+、hg2+等重金属离子对克氏原螯虾（procambarusclarkia）n-乙酰-β-d-氨基葡萄糖苷酶（nagase）活力的影响，结果表明：4种重金属离子对酶活力均有不同程度的可逆抑制作用，其中hg2+、zn2+对酶的抑制作用较强，表现为反竞争性抑制，对结合酶（es）的抑制常数kis分别为0.07mmol/l和22.04mmol/l；而ag+、pb2+对酶有先激活后抑制的作用，其中ag+对酶的抑制作用类型表现为反竞争性抑制，其抑制常数ki为1.08mmol/l，pb2+对酶的抑制作用类型表现为非竞争性抑制，其抑制常数ki为26.17mmol/l.图9参11
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