|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
一株抗肿瘤放线菌的鉴定及生物活性
DOI: 10.3724/SP.J.1145.2012.00983, PP. 983-987
Keywords: 青藏高原,青海湖沙岛,放线菌,砖红链霉菌,抗肿瘤,菌株鉴定,生物活性
Abstract:
为开发青藏高原沙地生态系统放线菌资源，首次从位于青藏高原东北部的青海湖沙岛土壤样品中选择性分离培养出一株具明显抑菌及抗肿瘤活性放线菌sd-31.通过生理生化特性、化学分类学以及16srrna序列分析鉴定，菌株为砖红链霉菌，命名为streptomyceslateritiussd-31；双层琼脂法检测菌株抑菌活性，表明菌株对金黄色葡萄球菌（staphyloccocusaureus）以及枯草芽孢杆菌（bacillussubtilis）有显著抑制作用；通过hoechst染色荧光显微镜观察及mtt法细胞毒性试验，确定菌株发酵液有明显的诱导肿瘤细胞凋亡活性且与处理浓度成正相关；纸片法研究菌株发酵液活性稳定性，结果表明，20~80℃处理后发酵液抑菌活性稳定，100℃条件处理后发酵液抑菌活性降低；在酸性及中性条件下发酵液活性稳定，碱性条件下随着ph值的增加发酵液抑菌活性降低.以上结果为抗肿瘤药物的研究提供了理论数据.图6表4参23
| [1] | 6tohtyd(迪丽拜尔?托乎提),habdinx(旭格拉?哈布丁),chenj(陈瑾),zhuyl(朱艳蕾),hasj(艾山江),andd(安登第),zhouyg(周宇光).biologicalcharacteristicsandactivesubstancesofalkalophilicactinomycetesfromextremeenvironmentoflopnurperipheralsinxinjiang,china.chinjapplenvironbiol(应用与环境生物学报),2009,15(6):835~839
|
| [2] | 7fanlx(范丽霞),zhengjp(郑继平),yangxh(杨先会).theeffectofnaturalair-drytimeonactinomycetesisolationfromsamplesoil.jhainanmedcoll(海南医学院学报),2010,16(3):280~284
|
| [3] | 20zhangyq,schumannp,yuly,liuhy,zhangyq,xulh,stackebrandte,jiangcl,liwj.zhihengliuellahalotoleransgen.nov.,sp.nov.,anovelmemberofthefamilymicrococcaceae.intjsystevolmicrobiol,2007,57:1018~1023
|
| [4] | 22gilpinml,boxsj,elsonal.newquinineantibioticsofthegranaticintype,isolatedfromstreptomyceslateritiusⅱ.structuredetermination.jantibiotics,1988,xli(4):512~518
|
| [5] | 23fleckwf,stranssdg,prauserh.naphthoquinoneantibioticsfromstreptomyceslateritius.ifermentation,isolationandcharacterizationofgranatomycinsa,candd.zallgmikrobiol,1980,20(9):543~551
|
| [6] | 1zhaok,penttinenp,guant,xiaoj,chenq,xuj,lindströmk,zhangll,zhangxp,strobelga.thediversityandanti-microbialactivityofendophyticactinomycetesisolatedfrommedicinalplantsinpanxiplateau,china.currmicrobiol,2011,62:182~190
|
| [7] | 2okorock,brownr,jonesal,andrewsba,asenjoja,goodfellowm,bullat.diversityofculturableactinomycetesinhyper-aridsoilsoftheatacamadeserts,chile.antonievanleeuwenhoek,2009,95:121~133
|
| [8] | 3jiangsm,sunw,chenmj,daisk,zhangl,liuyh,leekj,lix.diversityofculturableactinobacteriaisolatedfrommaringspongehaliclonasp.antonievanleeuwenhoek,2007,92(4):405~416
|
| [9] | 4zenovag.m,kurapovaai,lysenkoam,zvyagintsevdg.thestructural-functionalorganizationofthethermotolerantcomplexesofactinomycetesindesertandvolcanicsoils.eurasiansoilsci,2009,42(5):531~535
|
| [10] | 5simr(司美茹),xueqh(薛泉宏),chenzq(陈占全),yub(余博),caiy(蔡艳).ecologicaldistributionofsoilantimicrobialactinomycetesoftheqinghaiplateau.chinjapplenvironbiol(应用与环境生物学报),2005,11(1):104~111
|
| [11] | 8jiangy(姜怡),duansr(段淑蓉),tangsk(唐蜀昆),chenhh(陈华红),liwj(李文均),xulh(徐丽华).someapproachesfortheselectiveisolationofrareactinomycetes.microbiology(微生物学通报),2006,33(1):181~183
|
| [12] | 9jiangy(姜怡),caoyr(曹艳茹),zhaolx(赵立兴),wangq(王茜),lerx(勒荣线),hewx(和文祥),xueqh(薛泉宏).ultrasonictreatmentofsoilsamplesforactinomyceteisolation.actamicrobiolsin(微生物学报),2010,50(8):1094~1097
|
| [13] | 10staneckjl,robertsgd.simplifiedapproachtoidentificationofaerobicactinomycetesbythin-layerchromatography.applmicrobiol,1974,28(2):226~231
|
| [14] | 11ruanjs(阮继生),huangy(黄英).rapididentificationandsystematicsofactinobacteria.beijing,china:sciencepress(北京:科学出版社),2011.69~116
|
| [15] | 12staneckjl,robertsgd.simplifiedapproachtoidentificationofaerobicactinomycetesbythin-layerchromatography.applmicrobiol,1974,28(2):226~231
|
| [16] | 13xulh(徐丽华),liwj(李文均),liuzh(刘志恒),jiangcl(姜成林).actinomycetesystematic―principle,methodsandpractice.beijing,china:sciencepress(北京:科学出版社),2007.80~87
|
| [17] | 14heuerh,krsekm,bakerp,smallak,wellingtonemh.analysisofactinomycetecommunitiesbyspecificamplificationofgenesencoding16srrnaandgel-electrophoreticseparationindenaturinggradients.applenvironmicrobiol,1997,63:3233~3241
|
| [18] | 15tamurak,dudleyj,neim,kumars.mega4:molecularevolutionarygeneticsanalysis(mega)softwareversion4.0.molbiolevol,2007,24(8):1596~1599
|
| [19] | 16thompsonjd,gibsontj,plewniakf,jeanmouginf,higginsdg.theclustal_xwindowsinterface:flexiblestrategiesformultiplesequencealignmentaidedbyqualityanalysistools.nucleicacidsres,1997,25:4876~4882
|
| [20] | 17chenj,chenhm,zhangyq,weiyz,liqp,liuhy,suj,zhangyq,yuly.agromycesflavussp.nov.,anactinomyceteisolatedfromsoil.intjsystevolmicrobiol,2011,61(7):1705~1709
|
| [21] | 18zhangyq,liuhy,chenj,yuanlj,sunw,zhanglx,zhangyq,yuly,liwj.diversityofculturableactinobacteriafromqinghai-tibetplateau,china.antonievanleeuwenhoek,2010,98:213~223
|
| [22] | 19zhangyq,schumannp,liwj,chengz,tianxp,stackebrandte,xulh,jiangcl.isoptericolahalotoleranssp.nov.,anovelactinobacteriumisolatedfromsalinesoilfromqinghaiprovince,north-westchina.intjsystevolmicrobiol,2005,55:1867~1870
|
| [23] | 21tuntiwachwuttikulp,taechowisant,wanbanjoba,thadanitis,taylorwc.lansaiad,secondarymetabolitesfromstreptomycessp.suc1.tetrahedron,2008,54:7583~7586
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
