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OALib Journal期刊
ISSN: 2333-9721
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产酯酶b1海洋枯草芽孢杆菌c5发酵条件优化
DOI: 10.3724/SP.J.1145.2012.00999, PP. 999-1003
Keywords: 枯草芽孢杆菌,酯酶,发酵,优化,plackett-burman,响应面法,甲基对硫磷
Abstract:
为提高芽孢杆菌c5产酯酶b1的能力，采用响应面法对其发酵条件进行优化.首先通过单因素实验筛选氮源、碳源、接种量、起始发酵ph、装液量、发酵温度和转速这7个因素，当酶活达到最高值时，各单因素的值分别为氮源玉米浆30ml/l，碳源麦芽糖35g/l，接种量4%（φ），起始ph7.0，装液量15ml，培养温度36℃，转速在实验室现有条件下最高选择220r/min；再通过plackett-burman（pb）设计法，评价了这7个因素对酯酶b1产量影响的大小，确定氮源玉米浆的浓度、发酵起始ph和发酵温度为酯酶产生的3个主要影响因素；利用中心组合设计（ccd）及sas软件分析获得了主要因素的最优条件，即玉米浆浓度28.70ml/l，起始发酵ph为7.10，发酵温度为35.8℃.预测最高酶活力为139.18u/ml，实验最终酶活达到138.40u/ml，与原发酵条件相比提高了228.15%.其实验值与预测值基本相符，说明预测模型可应用于酯酶发酵条件的优化.图3表4参21
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