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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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colorimetricdeterminationofmonosodiumglutamateinfoodsamplesusingl-glutamateoxidase
DOI: 10.3724/SP.J.1145.2013.01069, PP. 1069-1072
Keywords: msg,colorimetry,l-glutamateoxidase,soup,noodle,hamburger
Abstract:
arapid,sensitiveandsimplechromogenicmethodwasdevelopedforquantitativedeterminationofmonosodiumglutamate(msg)infoodsamples.themethodincorporatedaglutamateoxidaseandperoxidase.thelibratedh2o2fromaglutamatesampleasaresultofenzymaticactionwasmeasuredusing4-aminoantipyrineandphenolasachromogenicreagentat502nm.glutamatecalibrationcurvewaslinearupto125mmoll-1withadetectionlimitof2mmoll-1.theresultsofanalyzingl-glutamateinvariousfoodsamplesusingtheapproachwerecomparedtothoseofastandardprocedureemployinghplcmethod.msgconcentrationindifferentsamplesrangedfrom0.93to4.9g/kg.thismethodisrelativelysensitiveandspecific,withouttheneedofpre-treatmentforsample.
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