|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
茎瘤芥pr4基因的克隆与表达分析
DOI: 10.3724/SP.J.1145.2013.00974, PP. 974-979
Keywords: 芸薹根肿菌,茎瘤芥,病程相关蛋白4,表达分析
Abstract:
病程相关蛋白（prs）在植物抗病过程中起着重要作用，pr4是prs家族中参与植物系统获得抗性的重要效应因子.利用race技术从受十字花科根肿菌plasmodiophorabrassicae侵染的茎瘤芥中克隆到一个pr4基因，命名为bjpr4（genbank登录号为jx467703），bjpr4基因核苷酸序列全长584bp，编码140个氨基酸；bjpr4蛋白具有高度保守的barwin结构，与其它植物的pr4蛋白具有较高的相似性，系统进化分析表明该蛋白基因在不同物种之间具有保守性.bjpr4序列中没有几丁质结合域结构，所以这个基因属于ⅱ类pr4.qrt-pcr检测分析显示，十字花科根肿菌处理后，bjpr4在病程初期表达量较低，病程后期表达量急剧上升；水杨酸处理后，bjpr4在短期内上调表达，12h表达量最高，随后降低至较低水平；而茉莉酸甲酯、乙烯、脱落酸处理后该基因表达水平均下降.研究结果表明bjpr4在茎瘤芥根部的表达受到不同植物信号分子的调控，bjpr4在根肿病病程中的表达特征表明bjpr4与茎瘤芥对根肿菌的抗性有关.
| [1] | 1彭洪江,王旭�,高明泉,韩海波,肖崇刚.涪陵榨菜上严重发生根肿病[j].植物保护,2002,28(2):59-60[penghj,wangxy,gaomq,hanhb,xiaocg.seriousclubrootexplosiveepiphytoticsinfulinstemmustardbrassicajunceavar.tumida[j].plantprot,2002,28(2):59-2liuy,yinyp,wangzk,luoyl.expressionofnitrilaseinbrassicajunceavar.tumidainrootgallscausedbyplasmodiophorabrassicae.jintegragric[j],2012,11(1):100-1083ludwigm,astrids.whatcanwelearnfromclubroots:alterationsinhostrootsandhormonehomeostasiscausedbyplasmodiophorabrassicae[j].eurjplantpathol,2008,121(3):291-3024dixongr.plasmodiophorabrassicaeinitsenvironment[j].jplantgrowthregul,2009,28(3):212-2285陈晓峰,侯喜林,刘琳.不结球白菜pr4蛋白基因的克隆与诱导表达分析[j].西北植物学报,2008,28(1):1-6[chenxf,houxl,liul.cloningandexpressioncharacterizationofpr4proteingeneinbrassicacampestrisssp.chinensis[j].actabotbor-occidsin,2008,28(1):1-6]6赵淑清,郭剑波.植物系统获得抗性及信号转导途径[j].中国农业科学,2003,36(7):781-787[zhaosq,guojb.systemicacquiredresistanceandsignaltransductioninplant[j].sciagrisin,2003,36(7):781-787]7vanloonlc,repm,pietersecmj.signi?canceofinducibledefense-relatedproteinsininfectedplants[j].annurevphytopathol,2006,44:135-1628王燕华,何水林.植物诱导抗病性的分子生物学研究进展[j].应用与环境生物学报,2004,10(6):811-815[wangyh,hesl.molecularbiologyresearchofplant-induceddiseaseresistance[j].chinjapplenvironbiol,2004,10(6):811-815]9dongx.geneticdissectionofsystemicacquiredresistance[j].curropinplantbiol,2001,4(4):309-31410vanloonlc,vanstrienea.thefamiliesofpathogen-esis-relatedproteins,theiractivities,andcomparativeanalysisofpr-1typeproteins[j].physiolmolplantpathol,1999,55:85-9711edrevaa.pathogenesis-relatedproteins:researchprogressinthelast15years[j].genapplplantphysiol,2005,31(1-2):105-12412ludvigsens,poulsenfm.three-dimensionalstructureinsolutionofbarwin,aproteinfrombarleyseed[j].biochemistry,1992,31(37):8783-878913lixd,xiab,jiangym,wuqs,wangcy,hels,pengf,wangr.anewpathogenesis-relatedproteinlrpr4fromlycorisradiataanditsantifungalactivityagainstmagnaporthegrisea[j].molbiolrep,2010,37(2):995-100114郭向华,肖崇刚,曾艳.甘兰根肿菌生物学特性[j].西南农业大学学报,2001,23(1):7-9[guoxh,xiaocg,zengy.biologicalcharacteristicsofplasmodiophorabrassicae[j].jsouthwestagricuniv,2001,23(1):7-9]15parkys,minhj,ryangsh,ohkj,chajs,kimhy,chotj.characterizationofsalicylicacidinducedgenesinchinesecabbage[j].plantcellrep,2003,21(10):1027-103416bertinil,leonardil,caporalec,tuccim,casconen,diberardinoi,buonocorev,carusoc.pathogen-responsivewheatpr4genesareinducedbyactivatorsofsystemicacquiredresistanceandwounding[j].plantsci,2003,164(6):1067-107817parkys,jeonmh,leesh,moonhs,chajs,kimhy,chotj.activationofdefenseresponsesinchinesecabbagebyanonhostpathogen,pseudomonassyringaepv.tomato[j].biochemmolbiol,2005,38(6):748-75418parksw,kaimoyoe,kumard,moshersf,klessigd.methylsalicylateisacriticalmobilesignalforsystemicacquiredresistance[j].science,2003,318(5847):113-11619koornneefa,leon-reyesa,ritsemat,verhagea,denotterfc,vanloonlc,pietersecmj.kineticsofsalicylate-mediatedsuppressionofjasmonatesignalingrevealaroleforredoxmodulation[j].plantphysiol,2008,147(3):1358-136820andersonjp,badruzsaufarie,schenkpm,mannersjm,desmondoj,ehlertc,macleandj,ebertpr,kazank.antagonisticinteractionbetweenabscisicacidandjasmonate-ethylenesignalingpathwaysmodulatesdefensegeneexpressionanddiseaseresistanceinarabidopsis[j].plantcell,2004,16(12):3460-3479
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
