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OALib Journal期刊
ISSN: 2333-9721
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基于响应面设计的百日咳粘附素浸提工艺优化
DOI: 10.3724/SP.J.1145.2013.01064, PP. 1064-1068
Keywords: 百日咳,百日咳粘附素,响应面,浸提
Abstract:
百日咳粘附素（prn）是无细胞百日咳疫苗的重要保护性抗原之一，优化其浸提工艺对提高prn产量、纯度乃至百日咳疫苗的质量都具有重要意义.通过中心复合表面设计（ccf）响应面模型和elisa定量分析相结合的方法研究了影响prn浸提工艺的关键因素.经两轮ccf响应面优化，确定了prn浸提的最优工艺参数：温度60℃，菌浓度50mg/ml，时间90min，ph8.5，nacl0.15mol/l；通过montecarlo模拟和plackettburman模型设计预测和验证了工艺稳健性参数范围：温度58-62℃，菌浓度40-60mg/ml，时间80-100min，ph8.45-8.55，nacl0.12-0.18mol/l.响应面优化后，粗提液prn产量提高了50%.研究表明，温度对prn浸提影响最大，其次是ph；菌浓度、时间及nacl含量的影响相对较小，但与温度、ph有交互作用.
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