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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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高效催化合成3-羟基丙酸的菌株特性
DOI: 10.3724/SP.J.1145.2014.03003, PP. 804-808
Keywords: acetobactersp.,1,3-丙二醇,3-羟基丙酸,催化特性
Abstract:
3-羟基丙酸（3-hp）是一种具有重要经济价值的新兴平台化合物，可用来合成多种化工产品和特种生物降解材料.本研究以1,3-丙二醇（1,3-pdo）为底物全细胞催化合成3-hp.通过菌株筛选和esi-ms验证共得到3株目的微生物，经鉴定比较，acetobactersp.催化合成3-hp性能最高，10gl-11,3-pdo经7h可生成11.65gl-1的3-hp，摩尔转化率高达98.4%.对acetobactersp.底物催化特异性研究表明该菌株对其他相关醇类如乙二醇、戊醇、苯甲醇、苯乙醇等具有不同程度的催化能力，acetobactersp.对甘油仅具有微弱催化活性.研究表明ca2+、mg2+、fe3+能够显著提高细胞催化合成3-hp的速率，向全细胞催化液中加入终浓度0.01moll-1的cacl2，细胞催化活性提高了11.6%.同时考察了氧载体和表面活性剂、贮存温度、菌体重复利用对acetobactersp.催化活性的影响并获得了该菌株催化合成3-hp的特性.利用acetobactersp.细胞催化1,3-pdo，为3-hp的制备提供了一种新的可能.
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