|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
5个杀虫调控基因对苏云金芽胞杆菌407解毒cr(ⅵ)的影响
DOI: 10.3724/SP.J.1145.2014.01023, PP. 785-790
Keywords: 苏云金芽胞杆菌,解毒,cr(ⅵ),cr(ⅵ)还原,cr(ⅵ)抗性,调控基因
Abstract:
测定苏云金芽胞杆菌（bacillusthuringiensis，简称bt）407及其突变株407δplcr、407δinha2、407δmpbe、407δsigmae和407δsigmak对数生长期与稳定期的拐点时间t0，测定不同时间点发酵液上清中的总铬（cr）和cr(ⅵ)的浓度，并分析bt407基因组的抗性相关基因，进而初步构建bt解毒cr(ⅵ)模式图.结果表明，plcr、inha2、mpbe、sigmak负调控bt407解毒cr(ⅵ)的能力并主要影响cr(ⅵ)还原过程，sigmae不影响对cr(ⅵ)的解毒过程.利用bt407基因组序列分析得到与cr(ⅵ)抗性相关基因nadph偶氮还原酶基因azor、外排蛋白基因efx和chra.推测bt407对cr(ⅵ)抗性机制可能为：azor编码nadph偶氮还原酶将细胞内cr(ⅵ)还原为cr(ⅲ)，cr(ⅲ)再通过chra或与azor共表达的外排蛋白efx从细胞质中排出，这一还原过程直接/间接受到plcr、inha2、mpbe、sigmak等调控基因的负调控.因此，bt407对cr(ⅵ)的解毒机制是由azor、efx、chra等主效基因与plcr、inha2、mpbe、sigmak等调控基因共同作用的复杂网络.
| [1] | 2feuchta,evansl,erringtonj.identificationofsporulationgenesbygenome-wideanalysisofthesigmaeregulonofbacillussubtilis[j].microbiology,2003,149(pt10):3023-3034
|
| [2] | 3李雪雁,李照会,许维岸.苏云金芽胞杆菌cry基因研究进展[j].昆虫知识,2003,40(1):9-13[liyy,lizh,xuwy.researchadvancesonthecrygenesofbacillusthuringiensis[j].entomolknowl,2003,40(1):9-
|
| [3] | 4agaisseh,gominetm,okstadoa,kolst?ab,lereclusd.plcrisapleiotropicregulatorofextracellularvirulencefactorgeneexpressioninbacillusthuringiensis[j].molmicrobiol,1999,32(5):1043-1053
|
| [4] | 5okstadoa,gominetm,purnelleb,rosem,lereclusd,kolst?ab..sequenceanalysisofthreebacilluscereuslocicarryingpicr-regulatedgenesencodingdegradativeenzymesandenterotoxin[j].microbiology,1999,145(pt11):3129-3138
|
| [5] | 13刘婉,李泽琴.水中铬污染治理的研究进展[j].广东微量元素科学,2007,9(14):5-9[liuw,lizq.progressonresearchoftreatingcrcontainingwastewater[j].guangdongweiliangyuansukexue,2007,9(14):5-
|
| [6] | 14马前,宋卫峰,吴斌,李义久,倪亚明.含cr（ⅵ）废水生物处理技术及其影响因素[j].四川环境,2001,4(20):19-22[maq,songwf,wub,liyj,niym.biologicaltreatingtechnologyanditsfactorsofcr(vi)-containingwastewater[j].sichuanenviron,2001,4(20):19-
|
| [7] | 15ramírez-díazmi,díaz-pérezc,vargase,riveros-rosash,campos-garcíaj,cervantesc.mechanismsofbacterialresistancetochromiumcompounds[j].biometals,2008,21(3):321-332
|
| [8] | 16ackerleydf,gonzalezcf,parkch,blaker2nd,keyhanm,matina.chromate-reducingpropertiesofsolubleflavoproteinsfrompseudomonasputidaandescherichiacoli[j].applenvironmicrobiol,2004,70(2):873-882
|
| [9] | 17汪频,李福德,刘大江.硫酸盐还原菌还原铬（ⅵ）的研究[j].环境科学,1993,14(6):1-4[wangp,lidf,liudj.studiesonremovinghexavalentchromiumbysulfate-reducingbacteria[j].chinjenvironsci,1993,14(6):1-
|
| [10] | 18brancor,chungap,johnstont,gurelv,moraisp,zhitkovicha.thechromate-induciblechrbacfoperonfromthetransposableelementtnotchrconfersresistancetochromium(vi)andsuperoxide[j].jbacteriol,2008,190(21):6996-7003
|
| [11] | 21gb7466-87.水质总铬的测定[s].[gb7466-87.waterquality-determinationoftotalchromium[s]]
|
| [12] | 22erringtonj.bacillussubtilissporulation:regulationofgeneexpressionandcontrolofmorphogenesis.microbiolrev,1993,57(1):1-33.
|
| [13] | 23bouillautl,perchats,arolds,zorrillas,slamtil,henryc,goharm,declerckn,lereclusd.molecularbasisforgroup-specificactivationofthevirulenceregulatorplcrbypaprheptapeptides[j].nucleicacidsres,2008,36(11):3791-3801
|
| [14] | 24declerckn,bouillautl,chaixd,ruganin,slamtil,hohf,lereclusd,aroldst.structureofplcr:insightsintovirulenceregulationandevolutionofquorumsensingingram-positivebacteria[j].procnatlacadsciusa,2007,104(47):18490-18495
|
| [15] | 25slamtil,lereclusd.specificityandpolymorphismoftheplcr-paprquorum-sensingsysteminthebacilluscereusgroup[j].jbacteriol,2005,187(3):1182-1187
|
| [16] | 26lereclusd,agaisseh,gominetm,salamitous,sanchisv.identificationofabacillusthuringiensisgenethatpositivelyregulatestranscriptionofthephosphatidylinositol-specificphospholipasecgeneattheonsetofthestationaryphase.jbacteriol,1996,178(10):2749-2756
|
| [17] | 29cervantesc,ohtakeh,chul,misratk,silvers.cloning,nucleotidesequence,andexpressionofthechromateresistancedeterminantofpseudomonasaeruginosaplasmidpum505[j].jbacteriol,1990,172(1):287-291
|
| [18] | 31张利莉,喻子牛.sigma因子和启动子上游区在苏云金芽胞杆菌杀虫晶体蛋白基因表达调控中的作用[j].中国生物工程杂志,2003,3(23):50-54[zhangll,xuzz.effectofσfactorsandpromoterupstreamregiononregulationofexpressionofinsecticidalcrystalproteingenesinbacillusthuringiensis[j].jchinbiotech,2003,3(23):50-
|
| [19] | 32hsuehyh,somerseb,lereclusd,wongac.biofilmformationbybacilluscereusisinfluencedbyplcr,apleiotropicregulator[j].applenvironmicrobiol,2006,72(7):5089-5092
|
| [20] | 33parkch,keyhanm,wielingab,fendorfs,matina.purificationtohomogeneityandcharacterizationofanovelpseudomonasputidachromatereductase[j].applenvironmicrobiol,2000,66(5):1788-1795
|
| [21] | 34gonzalezcf,ackerleydf,lynchsv,matina.chrr,asolublequinonereductaseofpseudomonasputidathatdefendsagainsth2o2[j].jbiolchem,2005,280(24):22590-22595
|
| [22] | 1王利平,代林远,李鹏.苏云金芽胞杆菌研究进展[j].中国畜牧兽医,2011,9(38):224-227[wanglp,lindy,lip.researchadvancesonbacillusthuringiensis[j].chinaanimhusbandvetmed,2011,9(38):224-
|
| [23] | 6goharm,faegrik,perchats,ravnums,?kstadoa,gominetm,kolst?ab,lereclusd.theplcrvirulenceregulonofbacilluscereus[j].plosone,2008,3(7):e2793
|
| [24] | 7fedhilas,goharm,slamtil,nelp,lereclusd.thebacillusthuringiensisplcr-regulatedgeneinha2isnecessary,butnotsufficientforvirulence[j].jbacteriol,2003,185(9):2820-2825
|
| [25] | 8fedhilas,nelp,lereclusd.theinha2metalloproteaseofbacillusthuringiensisstrain407isrequiredforpathogenicityininsectsinfectedviatheoralroute[j].jbacteriol,2002,184(12):3296-3304
|
| [26] | 9苏新华.苏云金芽胞杆菌处理含cr(vi)废水的研究[d].福州:福建农林大学,2011[suxh.treatmentofwastewatercontainingcr(ⅵ)withbacillusthuringiensis[d].fuzhou:fujianagricultureandforestry,
|
| [27] | 10黄天培,张巧铃,潘洁茹,苏新华,肖颖,赖小华,郭杨菁,杨晓伟,康榕,关雄.高效还原铬的苏云金芽胞杆菌菌株筛选[j].应用与环境生物学报,2010,16(6):879-882[huangtp,zhangql,panjr,suxh,xiaoy,laixh,guoyj,yangxw,kangr,guanx.screeningofbacillusthuringiensiswithhighchromiumreducingcapacity[j].chinjapplenvironmicrobiol,2010,16(6):879-
|
| [28] | 11kanmanip,aravindj,prestond.remediationofchromiumcontaminantsusingbacteria[j].intjenvironscitech,2011,9(1):183-193
|
| [29] | 12petrillifl,defloras.toxicityandmutagenicityofhexavalentchromiumonsalmonellatyphimurium[j].applenvironmicrobiol,1977,33(4):805-809
|
| [30] | 19hennekl,nakatsuch,thompsondk,konopkaae.high-levelchromateresistanceinarthrobactersp.strainfb24requirespreviouslyuncharacterizedaccessorygenes[j].bmcmicrobiol,2009,9:199
|
| [31] | 20gb7467-87.水质六价铬的测定二苯碳酰二肼分光光度法[s][gb7467-87.waterquality―determinationofchromium(vi)―1,5diphenylcarbohydrazidespectrophotometricmethod[s]]
|
| [32] | 27grandvaletc,gominetm,lereclusd.identificationofgenesinvolvedintheactivationofthebacillusthuringiensisinhametalloproteasegeneattheonsetofsporulation.microbiology,2001,147(pt7):1805-1813
|
| [33] | 28hajaij-ellouzem,fedhilas,lereclusd,nielsen-lerouxc.theenhancin-likemetalloproteasefromthebacilluscereusgroupisregulatedbythepleiotropictranscriptionalactivatorplcrbutisnotessentialforlarvicidalactivity[j].femsmicrobiollett,2006,260(1):9-16
|
| [34] | 30cervantesc,silvers.plasmidchromateresistanceandchromatereduction[j].plasmid,1992,27(1):65-71
|
| [35] | 35eswaramoorthys,poulains,hienerwadelr,bremondn,sylvestermd,zhangyb,berthomieuc,vanderlelied,matina.crystalstructureofchrr--aquinonereductasewiththecapacitytoreducechromate[j].plosone,2012,7(4):e36017
|
| [36] | 36cheungkh,gujd.mechanismofhexavalentchromiumdetoxificationbymicroorganismsandbioremediationapplicationpotential:areview[j].intbiodeterbiodegr,2007,59(1):8-15
|
| [37] | 37tsibakhashviliny,kalabegishvilitl,rcheulishvilian,ginturyen,lomidzelg,gvarjaladzedn,rcheulishvilioa,holmanhy.effectofzn(ii)onthereductionandaccumulationofcr(vi)byarthrobacterspecies[j].jindmicrobiolbiotechnol,2011,38(11):1803-1808
|
| [38] | 38niesdh,kochs,wachis,peitzschn,saiermhjr..chr,anovelfamilyofprokaryoticprotonmotiveforce-driventransportersprobablycontainingchromate/sulfateantiporters[j].jbacteriol,1998,180(21):5799-5802
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
