|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
铁皮石斛尿苷二磷酸葡萄糖焦磷酸化酶基因（ugp）的密码子偏好性分析
DOI: 10.3724/SP.J.1145.2014.03041, PP. 759-766
Keywords: 铁皮石斛,尿苷二磷酸葡萄糖焦磷酸化酶基因（ugp）,密码子偏好性,聚类分析
Abstract:
尿苷二磷酸葡萄糖焦磷酸化酶（udp-glucosepyrophosphorylase，ugpase）是糖代谢的主要酶类之一.为了解铁皮石斛（dendrobiumofficinale）尿苷二磷酸葡萄糖焦磷酸化酶基因（ugp）的密码子使用特性，运用chips、cusp和codonw在线程序分析自主克隆的铁皮石斛ugp基因（genbank登录号：kf711982.1）的密码子偏好性，并与其他物种的ugp基因以及基因组进行比较.结果显示，铁皮石斛ugp基因偏好使用a/t结尾的密码子，27种偏好密码子（rscu>1）中偏好性较强的有taa和ccu（rscu≥2）.与其他物种的ugp基因密码子使用对比后，发现不同物种ugp基因密码子使用偏好性存在一定差异.在9个所选物种的聚类分析中，发现基于ugp基因cds序列聚类的进化树比基于rscu的聚类更与传统的系统发育观点接近.与3种生物基因组密码子使用频率比较发现，与大肠杆菌基因组密码子使用频率差值较大的有23个，与酵母和拟南芥基因组差值较大的均有13个，这预示着铁皮石斛ugp基因在酵母和拟南芥中的表达效率较高.本研究结果为铁皮石斛ugp基因选择最佳的外源表达系统以及提高其表达水平奠定了前期的研究基础.
| [1] | 11sharppm,liw-h.codonusageinregulatorygenesinescherichiacolidoesnotreflectselectionfor‘rare’codons[j].nucacidres,1986,14(19):7737-7749
|
| [2] | 12sharppm,liwh.anevolutionaryperspectiveonsynonymouscodonusageinunicellularorganisms[j].jmolevol,1986,24(1-2):28-38
|
| [3] | 13sauk,guptask,saus,ghoshtc.synonymouscodonusagebiasin16staphylococcusaureusphages:implicationinphagetherapy[j].virusres,2005,113(2):123-131
|
| [4] | 14jiangy,dengf,wanghl,huzh.anextensiveanalysisontheglobalcodonusagepatternofbaculoviruses[j].archvirol,2008,153(12):2273-2282
|
| [5] | 15李平,白云凤,冯瑞云,王原媛,张维锋.籽粒苋苹果酸酶(nad-me)基因密码子偏好性分析.应用与环境生物学报,2011,17(1):12-17[lip,baiyf,fengry,wangyy,zhangwf.analysisofcodonbiasofnad-megeneinamaranthushypochondriacus[j].chinjapplenvironbiol,2011,17(1):12-17
|
| [6] | 16wangl,roossinckmj.comparativeanalysisofexpressedsequencesrevealsaconservedpatternofoptimalcodonusageinplants[j].plantmolbiol,2006,61(4-5):699-710
|
| [7] | 17刘庆坡,冯英,董辉.20个物种同义密码子偏好性的比较分析[j].西北农林科技大学学报:自然科学版,2004,32(7):67-71[liuqp,fengy,dongh.comparativestudiesonsynonymouscodonusagebiasintwentyspecies[j].northwesta&funiv(natscied),2004,32(7):67-
|
| [8] | 18赵耀,刘汉梅,顾勇,黄玉碧.玉米waxy基因密码子偏好性分析[j].玉米科学,2008,16(2):16-21[zhaoy,liuhm,guy,huangyb.analysisofcharacteristicofcodonusageinwaxygeneofzeamays[j].jmaizesci,2008,16(2):16-21
|
| [9] | 19liuq,dous,jiz,xueq.synonymouscodonusageandgenefunctionarestronglyrelatedinoryzasativa[j].biosystems,2005,80(2):123-131
|
| [10] | 20hiraokay,kawamatak,haraguchit,chikashigey.codonusagebiasiscorrelatedwithgeneexpressionlevelsinthefissionyeastschizosaccharomycespombe[j].genestocells,2009,14(4):499-509
|
| [11] | 21fedorova,saxonovs,gilbertw.regulartiesofcontext-dependentcodonbiasineukaryoticgenes[j].nucacidres,2002,30(5):1192-1197
|
| [12] | 24moriyamae,powellj.codonusagebiasandtrnaabundanceindrosophila[j].jmolevol,1997,45(5):514-523
|
| [13] | 27时慧,王玉,杨路成,丁兆堂.茶树抗寒调控转录因子ice1密码子偏好性分析[j].园艺学报,2012,39(7):1341-1352[shih,wangy,yanglc,dingzt.analysisofcodonbiasofthecoldregulatedtranscriptionfactorice1inteaplant[j].actahort,2012,39(7):1341-
|
| [14] | 28吴正常,王靖,赵乔辉,朱世平,訾臣,吴圣龙,包文斌.猪脂多糖结合蛋白基因(lbp)的密码子偏好性分析[j].农业生物技术学报,2013,21(10):1135-1144[wuzc,wangj,zhaoqh,zhusp,zic,wusl,baowb.analysisofcodonbiasoflipopolysaccharide-bindingproteingene(lbp)inpigs(susscrofa)[j].jagricbiotechnol,2013,21(10):1135-
|
| [15] | 1chiapelloh,olliviere,landès-devauchellec,nitschkép,rislerj-l.codonusageasatooltopredictthecellularlocationofeukaryoticribosomalproteinsandaminoacyl-trnasynthetases[j].nucacidres,1999,27(14):2848-2851
|
| [16] | 2mcinerneyjo.replicationalandtranscriptionalselectiononcodonusageinborreliaburgdorferi[j].procnatlacadsci,1998,95(18):10698-10703
|
| [17] | 3moriyamaen,hartld.codonusagebiasandbasecompositionofnucleargenesindrosophila[j].genetics,1993,134(3):847-858
|
| [18] | 4吴宪明,吴松锋,任大明,朱云平,贺福初.密码子偏好性的分析方法及相关研究进展[j].遗传,2007,29(4):420-426[wuxm,wusf,rendm,zhupy,hefc.theanalysismethodandprogressinthestudyofcodonbias[j].hereditas,2007,29(4):420-
|
| [19] | 5吴晓俊,刘涤,胡之璧.尿苷二磷酸葡萄糖焦磷酸化酶[j].植物生理学通讯,2000,36(3):193-200[wuxj,liud,huzb.udp-glucosepyrophosphorylase[j].plantphysj,2000,36(3):193-
|
| [20] | 6吴晓俊,刘涤,胡之璧.黄芪毛状根中尿苷二磷酸葡萄糖焦磷酸化酶活性与多糖含量关系的研究[j].上海中医药大学学报,2000,14(2):53-55[wuxj,liud,huzb.studyonrelationshipbetweenuridinediphosphateglucosepyrophosphorylaseactivityandpolysaccharidecontentinhairyrootofastragalusmembranaceus[j].jshanghaiunivtradchinmed,2000,14(2):53-
|
| [21] | 7赵洲,陈向东,王立华,王秋颖,兰进.灵芝子实体多糖积累和糖代谢相关酶的关系研究[j].中国农学通报,2012,28(22):253-257[zhaoz,chenxd,wanglh,wangqy,lanj.studiesonthecorrelationbetweenpolysaccharidesaccumulationandsugarmetabolizingenzymesinganodermalucidum[j].chinagricscibull,2012,28(22):253-
|
| [22] | 8张帆,钟威,穆虹,李刚.过量表达osugp2基因提高紫芝多糖含量[j].菌物学报,2011,30(3):442-452[zhangf,zhongw,muh,lig.increasingpolysaccharideyieldbyover-expressionofosugp2inganodermasinense[j].mycosystema,2011,30(3):442-
|
| [23] | 9wrightf.the‘effectivenumberofcodons’usedinagene[j].gene,1990,87(1):23-29
|
| [24] | 10guptas,bhattacharyyat,ghoshtc.synonymouscodonusageinlactococcuslactis:mutationalbiasversustranslationalselection[j].jbiomstrucdynam,2004,21(4):527-535
|
| [25] | 22石秀凡,黄京飞,柳树群,刘次全.人类基因同义密码子偏好的特征以及与基因gc含量的关系[j].生物化学与生物物理进展,2002,29(3):411-414[shixf,huangjf,liusq,liucq.thefeatureofsynonymouscodonbiasandgc-contentrelationshipinhumangenes[j].progrbiochembiophys,2002,29(3):411-
|
| [26] | 23孙铮,马亮,murphyr,张宪省,黄大卫.wolbachiapipientiswmel基因组水平上的密码子使用分析[j].中国科学c辑:生命科学,2009,39(10):948-953[sunz,mal,murphyr,zhangxs,huangdw.thecodonusageofwolbachiapipientiswmelgenome[j].scisinvitae,2009,39(10):948-
|
| [27] | 25christiansonml.codonusagepatternsdistortphylogeniesfromorofdnasequences[j].amjbot,2005,92(8):1221-1233
|
| [28] | 26郭秀丽,王玉,杨路成,丁兆堂.茶树cbf1基因密码子使用特性分析[j].遗传,2012,34(12):1614-1623[guoxl,wangy,yangyc,dingzt.analysisofcodonusefeaturesofcbfgeneincamelliasinensis[j].hereditas,2012,34(12):1614-
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
