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OALib Journal期刊
ISSN: 2333-9721
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高效转化胆固醇菌株的筛选、鉴定及转化条件优化
DOI: 10.3724/SP.J.1145.2014.00101, PP. 101-106
Keywords: 胆固醇,4-胆甾烯-3-酮,胆固醇氧化酶,红球菌,植物甾醇
Abstract:
胆固醇由甾体母核和侧链构成，是一种新的甾体资源.以胆固醇为唯一碳源的选择培养基，利用富集培养法从土壤中筛选出一株细菌，编号5901.该菌能够快速高效转化胆固醇，专一性生成4-胆甾烯-3-酮.经形态学观察和16srdna序列比对分析，该菌株为rhodococcussp.该菌株转化胆固醇的最优条件为：生长温度和转化温度均为30℃，初始ph8.0.胆固醇质量浓度为5g/l，反应24h后，胆固醇转化率为95%.本研究表明，5901菌株较之前报道的菌株，底物浓度高，转化速率快，也可以催化其它甾体化合物的3β-羟基氧化和5位双键位移反应，具有潜在的研究和应用价值.
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