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OALib Journal期刊
ISSN: 2333-9721
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两株嗜热异养氨氧化细菌的鉴定及其氨氧化特性
DOI: 10.3724/SP.J.1145.2014.00157, PP. 157-161
Keywords: 异养氨氧化细菌,嗜热菌,菌株分离鉴定
Abstract:
为寻找适用于堆肥的嗜热异养氨氧化细菌，采用稀释涂布平板法和格里斯试剂显色法筛选嗜热异养氨氧化细菌，利用含nh4+培养基研究其最佳生长温度、ph条件以及其氨氧化特点和no2-、no3-的生成速率，并通过16srdna测序及blast比对确定其种属.结果显示，从大理洱源热泉中分离了两株嗜热异养氨氧化细菌菌株nc8和nj26；这两株菌的最适生长温度为55℃，最适生长ph为8.0；在最适生长条件下，nc8和nj26菌株对nh4+的最大单位体积减少率分别为1033.3mg/l和1183.67mg/l；对no2-和no3-最大单位体积产生率分别为23.79mg/l、272.30mg/l和33.81mg/l、366.70mg/l；种属鉴定表明，nc8和nj26分别为bacillus属的不同种.本研究表明，nc8和nj26为异养型氨氧化细菌，能将nh4+氧化成no2-和no3-，以no3-为主要积累产物，因此有望应用于堆肥过程中以减少氨气排放，同时提高堆肥中氮素的含量.
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