|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
低温纤维素降解菌的分离与鉴定
DOI: 10.3724/SP.J.1145.2014.00152, PP. 152-156
Keywords: 低温,纤维素降解菌,菌株分离,菌株鉴定
Abstract:
对内蒙古部分地区土壤中低温降解纤维素的微生物进行研究，以期获得一些高酶活的低温纤维素酶产生菌.采用纯培养的方法，在10℃下培养获得纯培养物.以细菌16srdna通用引物pcr扩增后进行序列同源性比对确定种属.以dns法测定纤维素酶活性，并对酶活较高的菌株进行产酶条件的优化.结果共分离得到55株可低温降解纤维素的菌株，16srdna序列分析表明它们分别属于γ-变形菌纲（γ-proteobacteria）、硬壁菌门（firmicutes）、放线菌门（actinobacteria）、拟杆菌门（bacteroidetes）以及β-变形菌纲（β-proteobacteria）.该55株菌的纤维素酶活性均在22℃下最高.其中菌株cf11在10℃下的酶活在分离得到的55株细菌中最高.通过优化，菌株cf11产纤维素酶的最佳条件初步确定为ph值为6.5，培养时间为10d，并且是以酵母提取物作为氮源，其纤维素酶活为58.091iu.因此菌株cf11是一株极具开发潜力的低温纤维素酶产生菌.
| [1] | 1.钱林,郑巧利,付瑾,王慧萍,郑春丽,柳建设.一株高效纤维素降解菌株的分离鉴定及其酶学性质[j].微生物学通报,2010,37(4):524-528[qianl,zhengql,fuj,wanghp,zhengcl,liujs.isolationandidentificationofacellulosedegradingbacteriumanditscellulasecharacterization[j].microbiolchina,2010,37(4):524-
|
| [2] | 4.gounot,am,russellnj.physiologyofcold-adaptedmicroorganisms[a].heidelberg,berlin:springer,1999.33-35
|
| [3] | 5.陈亮,迟乃玉,张庆芳.低温纤维素酶菌株cny086选育及发酵培养基优化(ⅰ)[j].微生物学通报,2009,36(10):1547-1552[chenl,chiny,zhangqf.breedingandfermentationmediumoptimizationofcold-activecellulasestraincny086(ⅰ)[j].microbiolchina,2009,36(10):1547-1552
|
| [4] | 6.侯运华.适冷海洋青霉纤维素酶和半纤维素酶的分子生物学研究[d].济南:山东大学,2006[houyh.molecularresearchonthecellulaseandhemicellulaseofanovelcold-adaptivemarinepencilliumfs010[d].jinan:shandonguniversity,
|
| [5] | 7.曾胤新,俞勇,陈波,李会荣.低温纤维素酶产生菌的筛选、鉴定、生长特性及酶学性质[j].高技术通讯,2005,15(4):58-62[zengyx,yuy,chenb,li,hr.screening,classificationandgrowthofbacteriumproducingcold-activecellulaseanditsenzymiccharacterization[j].chinhightechnollett,2005,15(4):58-
|
| [6] | 10.侯进慧,孙会刚,郑宝刚,田力,蔡侃.一株产低温纤维素酶细菌的初步分析[j].生物学通报,2010,45(8):44-46
|
| [7] | 11.胡晓红,彭惠民,刘昕,黄正根,袁科.pcr及real-timepcr评价细菌dna提取方法[j].重庆医科大学学报,2008,33(2):155-158[huxh,penghm,liux,huangzg,yuank.methodsofdnaextractionfrombacteriaforpcrandreal-timepcr[j].jchongqingmeduniv,2008,33(2):155-
|
| [8] | 12.卢月霞,陈凯.纤维素降解菌的筛选及相互作用分析[j].安徽农业科学,2007,35(1):11-17[luyx,chenk.screeningofcellulosedecompositionmicroorganismsandanalysisoftheirreciprocity[j].janhuiagricsci,2007,35(1):11-
|
| [9] | 13.隋天娥,陶征义.低温微生物的适冷性及其应用[j].河北化工,2009,32(2):8-9[suite,taozy.thecold-adaptedmechanismandapplicationofcold-adaptedmicroorganisms[j].hebeichemind,2009,32(2):8-
|
| [10] | 14.高文峰,武伟男.低温微生物及其在环境工程中的应用[j].环境保护与循环经济,2010(4):50-52
|
| [11] | 15.刘尧.玉米秸秆腐解复合菌系css-1的构建、菌群组成及腐解特性研究[d].泰安:山东农业大学,2010[liur.construction,microbialcommunityanddecompositioncharacteristicsforthecomplexmicrobialsystemcss-1ofhighdecompositionefficiencyforcornstalks[d].tai’an:shandongagriculturaluniversity,
|
| [12] | 16.张丹,许景钢,路伟明.低温降解纤维素的细菌的筛选及鉴定[j].东北农业大学学报,2008,39(1):55-57[zhangd,xujg,luwm.selectionandidentificationofbacteriadegradingtextilefibersunderlowtemperature[j].jnortheastagricuniv,2008,39(1):55-
|
| [13] | 17.董硕.产低温纤维素酶菌株筛选及发酵条件研究[d].大连:大连大学,2011[studyonthescreeningandfermentationconditionsofanovelcold-activecelluloseproducingstrain[d].dalian:dalianuniversity,
|
| [14] | 19.margesnr,schnnerf.lowtemperaturebioremediationofawatercontaminatedwithanionicsurfactantsandfueloil.applmicrobiolbiotechnol,1998,49(4):482-486
|
| [15] | 20.fellerg,gerdayc.psychrophilicenzyme:molecularbasisofcoldadaptation[j].cellmollifesci,1997,53(10):830-841
|
| [16] | 21.陆小波,魏云林.低温酶及其冷适应性机制研究进展[j].生物技术通报,2006,16(4):52-53[luxb,weiyl.progressinthestudyofcold-adaptedenzymeandtheircold-adaptationmechanisms[j].biotechnolbull,2006,16(4):52-
|
| [17] | 2.魏艳红,熊鹰,袁永泽,郑扬忠,胡柳如,张华,李风,杨江科,闫云君,刘德立.纤维素酶产生菌hs-f9的筛选鉴定和产酶条件优化[j].应用与环境生物学报,2010,16(2):274-278[weiyh,xiongy,yuanyz,zhengyz,hulr,zhangh,lif,yangjk,yanyj,liudl.screeningandidentificationofacellulase-producingfungusandoptimizationofitsfermentationconditions[j].chinjapplenvironbiol,2010,16(2):274-
|
| [18] | 3.林艳梅,生吉萍,申琳,程凡升.适冷纤维素降解微生物研究进展[j].生物技术,2010,20(2):95-98[linym,shengjp,shenl,chengfs.trendsincold-adaptedandcellulose-degradingmicroorganisms.biotechnology,2010,20(2):95-
|
| [19] | 8.游银伟,汪天虹.适冷海洋细菌交替假单胞菌(pseudoalteromonassp.)mb-l内切葡聚糖酶基因的克隆和表达[j].微生物学报,2005,45(1):142-144[youyw,wangth.cloningandexpressionofendoglucanaseofmarinecold-adaptedbacteriapseudoalteromonassp.mb-1[j].actamicrobiolsin,2005,45(1):142-
|
| [20] | 9.danghy,zhuh,wangj,litg.extracellularhydrolyticenzymescreeningofculturableheterotrophicbacteriafromdeep-seasedimentsofthesouthernokinawatrough[j].worldjmicrobiolbiotechnol,2009,25(1):71-79
|
| [21] | 18.金城.低温纤维素酶的研究与生产[j].微生物学通报,2011,38(7):1140[jinc.researchandproductionofcold-activecellulase[j].microbiolchina,2011,38(7):
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
