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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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小麦矮秆基因rht-b1b、rht-d1b、rht8的检测及其对株高的影响
DOI: 10.3724/SP.J.1145.2014.00080, PP. 80-86
Keywords: 小麦,矮秆基因,xgwm261位点,致矮力
Abstract:
为进一步明确矮秆基因对杂交后代的致矮能力，一方面利用分子标记对26份小麦材料中所含有的矮秆基因（rht-b1b、rht-d1b、rht8）进行了检测，另一方面选取较矮的8份材料作为母本，与两个高秆地方品种（中国春、宜宾白麦子）进行杂交，测量f1代株高并计算致矮力；最后，结合两方面的数据来估计矮秆基因对株高的影响.结果表明，同一母本与不同父本，或同一父本与不同的母本，杂交后f1代株高存在较大差异.因此，各矮秆基因表达可能具有遗传背景效应.在与rht8紧密连锁的分子标记xgwm261位点扩增出了3个等位变异，分别为174bp、192bp和210bp，其中210bp片段所占比例最高，为57.7%.特别值得注意的是，检测到1份同时含rht-b1b＋rht-d1b＋rht8的材料（川育925矮）.由于其株高矮（57.92cm），平均致矮力高（29.4%），具高抗条锈病，且其它综合农艺性状好，因此推荐作为小麦矮化、半矮化育种的主要亲本之一.
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