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硫酸亚铁促进clostridiumacetobutylicumcicc8012发酵麦秸水解液产丁醇

DOI: 10.3724/SP.J.1145.2014.04012, PP. 447-454

Keywords: 丙酮丁醇梭菌,纤维素水解液,硫酸亚铁,原位脱毒,燃料丁醇

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Abstract:

以农业废弃物小麦秸秆为原料,以丙酮丁醇梭菌clostridiumacetobutylicumcicc8012为菌种发酵生产丁醇,通过单因素实验和响应面优化设计,优选硫酸亚铁对水解液进行原位脱毒,优化发酵条件并验证硫酸亚铁原位脱毒对多种原料的有效性.发酵结果显示,伴随发酵液残糖含量显著降低,溶剂产量和发酵效率显著提高.在优化的发酵条件下,初始糖浓度50g/l,接种量10.5%,硫酸亚铁浓度0.4g/l,发酵65h,丁醇产量达7.33g/l,与优化模型预测值7.35g/l的最大丁醇产量接近,模型显著可靠.对稻草水解液、玉米秸秆水解液、标准水解液等不同原料进行处理,丁醇和总溶剂产量均得到显著提高.本研究证明直接添加硫酸亚铁对秸秆水解液丁醇发酵具有显著促进作用,很可能是一种潜在的通用增效方法,可用于提高纤维素水解液的丁醇产量.

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