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OALib Journal期刊
ISSN: 2333-9721
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强化丙酮酸-co2途径对克雷伯氏菌合成1,3-丙二醇的影响
DOI: 10.3724/SP.J.1145.2014.12020, PP. 441-446
Keywords: 克雷伯氏菌,甲酸脱氢酶,丙酮酸甲酸裂解酶,1,3-丙二醇
Abstract:
克雷伯氏菌（klebsiellapneumoniae）中辅酶nadh的有效供给影响1,3-丙二醇的产量和得率，通过在k.pneumoniae强化丙酮酸-co2途径，以提高胞内nadh水平和1,3-丙二醇产量.将来源于酿酒酵母（saccharomycescerevisiaew303）的甲酸脱氢酶基因fdh1和k.pneumoniae的丙酮酸甲酸裂解酶基因pflb在k.pneumoniae中过表达，强化丙酮酸-甲酸-co2途径.结果显示，单独表达fdh1、pflb和共表达fhd1、pflb基因的克雷伯氏菌与出发菌株相比，胞内nadh含量明显增加，1,3-丙二醇产量分别提高了8%、12%、18%，摩尔转化率分别提高了7.3%、13.2%、19.5%，除乙酸外其他副产物都有不同程度的降低.本研究表明，强化丙酮酸-甲酸-co2途径提高了nadh的再生效率，促进了1,3-丙二醇的合成.
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