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OALib Journal期刊
ISSN: 2333-9721
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浮萍转录组数据ssr位点的生物信息学分析
DOI: 10.3724/SP.J.1145.2014.04027, PP. 401-405
Keywords: 浮萍,转录组,ssr,生物信息学
Abstract:
为开发浮萍分子标记技术，采用高通量测序技术获得浮萍转录组原始实验数据，经过生物信息学软件trinity拼接后，获得74797条unigene.进一步采用生物信息学分析软件misa对所有的unigene进行ssr位点数据挖掘.共计搜索出14250个ssr位点，分布在12707条unigene中，出现频率为19.05%，ssr平均长度为19bp，平均分布频率是1/6.57kb.在浮萍转录组的ssrs中，二核苷酸与三核苷酸重复序列为主要重复类型，分别占总ssrs的70.74%和25.37%.浮萍转录组数据中ssr序列共包括185种重复基元类型，二核苷酸重复基元ag/ct和三核苷酸重复基元ccg/cgg是优势重复基元，分别占总ssrs的69.19%和8.84%.综上表明浮萍ssrs位点具有极大的可开发性.
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