|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
多能硫杆菌1,5-二磷酸核酮糖羧化酶/加氧酶的酶学特征及基因检测
, PP. 361-365
Keywords: 多能硫杆菌,1,5-二磷酸核酮糖羧化酶/加氧酶,southern杂交
Abstract:
从固体平板的生长情况及rubisco酶活性测定结果,表明多能硫杆菌是通过卡尔文循环固定的co2,并具有该循环的关键酶──1,5-二磷酸核酮糖羧化酶/加氧酶.进一步将多能硫杆菌染色体dna用各种限制性内切酸酶切,southern转移到硝酸纤维素滤膜上,与光合细菌rhodobactersphaeroides的rubisco基因(rbcl-rbcs)探针杂交,显示出杂交带型,说明多能硫杆菌具有r.sphaeroides同源的羧化酶基因,并初步将该基因定位在3.0kb的pstⅰ片段内.
| [1] | kusanot,sugawarak,inouecetal.molecularcloningandexpressionoftaiobaciuusferooxidaxschromosomalribulosebisphosphatecarboxylasegenesinescherichiacoli,currentmicrobiology,1991,22:35~41
|
| [2] | sambrookj,fritschef.maniatist.molecularcloning,alaboratorymanual.2nded.newyork;coldspringharborlaboratorypress.1989
|
| [3] | holtjg,kriegnr,sneathphfetal.bergey’smanualofdeterminantivebacteriology.9thed.baltimore;thewilliams&wilkinsco.1992
|
| [4] | 刘振盈,颜望明.自养细菌rubisco分子生物学研究进展.微生物学通报,1993.20(2);110~119
|
| [5] | tabitafr.molecularandcellularregulationofautotrophiccarbondioxidefixationinmicroorganisms.microdiolrev.1988.52(2):155~189
|
| [6] | smithal.kellydp.woodap.metabolismofthiobacillusa2growthunderautotrophiic,mixotrophicandheterotrophicconditionsinchemastatculture.jcenmicrobial,1980.121:127~138
|
| [7] | robertcqjr.tabitafr.cloningandexpressioninescherecletacoleoftheformiribulose1,5-bisphosphatecarboxylase/oxygenasegenefromrhodopseudomonassphaeroides.gene.1984.31:91~101
|
| [8] | 刘振盈,颜望明.氧化亚铁硫杆菌tf-55基因文库的构建.山东大学学报(自然科学版).1993.28(3);358~369
|
| [9] | 北京大学生物系生物化学教研室编.生物化学实验指导.高等教育出版社,1979
|
| [10] | tabitafr.carusop,whitmanw.facileassayofenzymeuniquetothecalivincycleinintactcells.withspecialreferencetoribulose1,5-bisphophatecarboxylase.analyticalbiochernstry.1978.84;962~472
|
| [11] | gibsonjl.tabitafr.differentmolecularformsofribulose1,5-bisphosphatecarboxylasefromrhodopseudomonassphaerondes.jbiolchem.1977.252:943~949
|
| [12] | fuchsg,stupperiche,edeng.autotrophicco2fixationinclelarobivmlvmicola.evidencefortheoperationofareductivetricarboxylicacidcycleingrowingcells.archmicrobiol.1980.128:64~71
|
| [13] | andersonl,fullerrc.photosynthesisinrhodospirillumrubrvm.ⅱ.metaboliccontrolofreductivepentosephosphateandtricar-boxylicacidcycleenzyme.plantpdyseol.1967,42:497~502
|
| [14] | shibah,kawasumit,igarashiyetal.theco2assimilationviathereductivetricarboxylicacidcycleinaobligatelyautotropghic,aerobichydrogen-oxidizingbacteriumhydrogenobacterthermophilus.archmicrobiol.1985.141:198~203
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
