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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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紫云英根瘤菌共生基因分布及其共生效应的多样性
, PP. 49-54
Keywords: 紫云英根瘤菌,并生基因,并生效应
Abstract:
从同一植株不同根瘤分离40株紫云英根瘤菌,所有菌株对10种抗生素的抗药性测定表明,该群体分为22个抗药类群,质粒检测显示所有公离株都含有质粒,质粒数1～4条,用快生型大豆根瘤菌usda205质粒作参考,估测质粒mt分布范围为83～226mu.根据图谱分析表明,该菌群可分为6个不同质粒型.各类型质粒通过与dig-nodabc和dig-nifhdk杂交,结果显示带有1条质粒的菌株其共生基因定位在染色体上.带有2条或2条以上质粒的菌株各拥有1条共生质粒,共生质粒mr范围有差异,大约为117～220mu.研究结果也显示,不同质粒型的菌株其共生效应存在明显差异,其中第6质粒型的菌株共生固氮率最强,第1质粒型菌株共生固氮率较低.共生固氮能力最强的第6质粒型菌株,只占总菌数7.5%.
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