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OALib Journal期刊
ISSN: 2333-9721
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丛枝菌根真菌gigasporamargarita孢子伴生菌群效应分析
DOI: 10.3724/SP.J.1145.2008.00798, PP. 798-802
Keywords: 珍珠巨孢囊霉,孢子,伴生细菌,效应,丛枝菌根真菌
Abstract:
丛枝菌根真菌的孢子表面存在与之伴生的微生物类群，其中以细菌为主.本文以不同处理方式的珍珠巨孢囊霉(gigasporamargarita)孢子与白三叶草(trifoliumrepensl.)进行共培养试验.试验分为4组：a，孢子经表面消毒；b，孢子表面消毒后并回接伴生菌；c，孢子未经表面消毒；d，孢子存在于土壤接种物中.以灭菌的蛭石为基质，修改的hoagland营养液作为补充营养，在相对无杂菌的环境中培养3mo，并取样观察.结果表明，处理a的植株茎叶鲜(干)重显著低于其它处理，根系鲜重无显著差异.菌根侵染率以d处理最高，a与b、c之间存在不显著的差异.产孢数量以a最少，并与b、c之间存在显著性差异.以pcr-dgge技术分析各处理之间孢子伴生细菌的种群差异.结果显示，a处理与其它处理间存在较大的差异，其它处理间差异较小.分析认为，伴生菌群对g.margarita及其宿主植物的生命活动起着直接或间接的影响.图1表3参26
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