|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
有机溶剂和变性剂对枯草芽胞杆菌溶栓酶活性的影响
DOI: 10.3724/SP.J.1145.2008.00825, PP. 825-829
Keywords: 溶栓酶,有机溶剂,变性剂,溶血栓
Abstract:
以枯草芽胞杆菌ld-8547菌株发酵液为材料，提取分离溶栓酶，研究了几种有机溶剂及变性剂对枯草杆菌溶栓酶活性的影响.结果表明，甲醇对溶栓酶表现为抑制作用，在10%~15%浓度范围时，其抑制作用最强；乙醇在5%~15%浓度范围内对酶活有激活作用，但随浓度的增大则转变为较强的抑制作用；正丁醇和异丙醇对酶活力则表现出强烈的抑制作用；丙三醇对酶活力有轻微的抑制作用，随着浓度增加抑制作用逐渐增强；异戊醇在5%~15%浓度范围内有一定的激活作用，但当浓度大于15%时则表现为抑制作用.醛类对酶活也有较强的抑制作用；丙酮、dmso、dmf、sds和异氰硫酸胍对酶活均有不同程度的抑制作用；脲浓度<0.01mmol/l时略有激活作用，随浓度增大转变为抑制作用.实验同时对edta在不同浓度和不同时间内对酶活力的影响进行了研究，结果表明，浓度<2mmol/l的edta基本不影响酶活力，但随着浓度的增大，其抑制作用明显增大.本实验还以牛血为材料，对酶液进行了体外抗血凝和溶血栓实验，结果表明，该酶具有抗血凝和溶血栓效果.图2参13
| [1] | 6pengy,yangx,zhangyz.microbialfibrinolyticenzymes:anoverviewofsources,production,properties,andthrombolyticactivityinvivo.applmicrobiolbiotechnol,2005,69:126~132
|
| [2] | 7pengy,zhangyz.optimizationoffermentationconditionsofdouchifibrinolyticenzymeproducedbybacillusamyloliquefaciensdc-4.chinfoodfermentind,2002,28:19~23
|
| [3] | 8liuj,xingj,changt,maz,liuh.optimizationofnutritionalconditionsfornattokinaseproductionbybacillusnattonlsseusingstatisticalexperimentalmethods.procbiochem,2005,40:2757~2762
|
| [4] | 9huangxh(黄小红),chenqx(陈清西),wangj(王君),shal(沙莉),huangzp(黄志鹏),guanx(关雄).effectsofdifferentorganicsolventsonthechitinasefrombacillusthuringenesis.chinjapplenvironbiol(应用与环境生物学报),2005,11(1):71~73
|
| [5] | 10zhangjp(张继平),chenqx(陈清西).inactivationkineticsofn-acetyl-β-d-glucosaminidasefromscyllaserratabydimethylsulfoxide.joceanogrtaiwanstrait(台湾海峡),2006,25(1):19~24
|
| [6] | 11yoshihikom,ryotaroh,akirah.characterizationofpolymer�enzymecomplexasanovelbiocatalystfornonaqueousenzymology.jmolcatalb:enzymatic,2003,22:79~88
|
| [7] | 12zhengzl,zuozy,liuzg.constructionofa3-dmodelofnattokinase,anovelfibrinolyticenzymefrombacillusnatto:anovelnucleophiliccatalyticmechanismfornattokinase.jmolgraph,2005,23:373~380
|
| [8] | 1wangct,jibp,lib,noutr,lipl,jih,chenlf.purificationandcharacterizationofafibrinolyticenzymeofbacillussubtilisdc33,isolatedfromchinesetraditionaldouchi.jindmicrobiolbiotehnol,2006,33(9):750~758
|
| [9] | 2wangsh,zhangc,yangyl,diaom,baimf.screeningofahighfibrinolyticenzymeproducingstrainandcharacterizationofthefibrinolyticenzymeproducedfrombacillussubtilisld-8547.worldjmicrobiolbiotechnol,2008,24(4):475~482
|
| [10] | 3kimsb,leedw,cheinghci,choeea,leesj,hongyh,choihj,pyunyr.purificationandcharacterizationofafibrinolyticsubtilisin-likeproteaseofbacillussubtilistp-6fromanindonesianfermentedsoybean,tempeh.jindmicrobiolbiotechnol,2006,33:436~444
|
| [11] | 4paikhd,leesk,heos,kimsy,leeh,kwontj.purificationandcharacterizationofthefibrinolyticenzymeproducedbybacillussubtiliskck-7fromchungkookjang.jmicrobiolbiotechnol,2004,14(4):829~835
|
| [12] | 5liucg(刘晨光),wangp(王鹏),wans(万顺).theprofileandprogressofmicrobialfibrinolyticenzyme.microbiology(微生物学通报),2002,29(2):46~49
|
| [13] | 13zhangrh,xiaol,pengy,wanghy,baif,zhangyz.geneexpressionandcharacteristicsofanovelfibrinolyticenzyme(subtilisindfe)inescherichiacoli.lettapplmicrobiol,2005,41:190~195
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
