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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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小麦tamyb2基因转化拟南芥表达载体的构建与遗传转化
DOI: 10.3724/SP.J.1145.2008.00750, PP. 750-752
Keywords: tamyb2-ⅰ,tamyb2-ⅱ,拟南芥,表达载体,遗传转化
Abstract:
采用双酶切方法构建了小麦tamyb2基因转化拟南芥表达载体pchf3-35sp-tamyb2-ⅰ-noster和pchf3-35sp-tamyb2-ⅱ-noster，并通过农杆菌gv3101介导，用渗透法将tamyb2-ⅰ和tamyb2-ⅱ基因转入拟南芥中.结果表明，tamyb2-ⅰ、tamyb2-ⅱ和表达载体pchf3分别被kpnⅰ和pstⅰ成功酶切为837bp、840bp和10.4kb的目标条带；分别将pchf3-35sp-tamyb2-ⅰ-noster和pchf3-35sp-tamyb2-ⅱ-noster转入农杆菌gv310l中，在提取的农杆菌质粒dna中分别扩增出了837bp和840bp目标条带，说明可用于拟南芥的转化；在含kan(50mg/l)的1/2ms培养基上筛选转基因当代拟南芥植株的种子(t0)，获得了t1代抗性植株，转化率约为0.1%.图3参14
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