|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
杆状病毒包涵体蛋白的共同抗原性
DOI: 10.3724/SP.J.1145.2008.00787, PP. 787-792
Keywords: 杆状病毒,棉铃虫核型多角体病毒,包涵体蛋白,保守多肽,westernblot,共同抗原性
Abstract:
在比对已公布的37种杆状病毒包涵体蛋白氨基酸序列的基础上，选定棉铃虫核型多角体病毒(hanpv)多角体蛋白的两个高度保守多肽(54-113aa和206-245aa)制备多克隆抗体，用westernblot法检测这两个高度保守多肽与多种杆状病毒包涵体蛋白之间的血清学关系.结果表明，hanpv多角体蛋白的两个多肽的抗体与14种核型多角体病毒的多角体蛋白和5种颗粒体病毒的颗粒体蛋白均有明显的杂交信号，表明杆状病毒的包涵体蛋白之间具有共同的抗原决定簇.根据杆状病毒包涵体蛋白之间的这种血清学关系，进一步讨论了利用免疫金试纸技术检测病毒杀虫剂中包涵体含量的可行性.图7参9
| [1] | 1blackbc,brennanla,dierkspm,gardie.commercializationofbaculoviralinsecticides.in:millerlked.thebaculoviruses.newyorkandlondon:plenumpress,1997
|
| [2] | 2sunxl(孙修炼),penghy(彭辉银).recentadvancesinbiologicalcontrolofpestinsectsbyusingvirusesinchina.virolsin(中国病毒学),2007,22:158~162
|
| [3] | 3zanottopm,kessingbd,maruniakje.phylogeneticinterrelationshipamongbaculoviruses:evolutionaryratesandhostassociation.jinvertebrpathol,1993,62:147~162
|
| [4] | 4cowanp,bulachd,goodgek,robertsona,tribede.nucleotidesequenceofthepolyhedrosisgeneregionofhelicoverpazeasinglenucleocapsidnuclearpolyhedrosisvirus:placementofthevirusinlepidopteranuclearpolyhedrosisvirusgroupii.jgenvirol,1994,75:3211~3218
|
| [5] | 5wangg(王根),zhangcx(张传溪),gongcl(贡成良),jinw(金伟),wuxf(吴祥甫).cloningandsequencingofhelicoverpaarmigeranuclearpolyhedrosisviruspolyhedrongene.chinjvirol(病毒学报),1997,13(1):83~87
|
| [6] | 6liuzy(刘子夜),qiyp(齐义鹏),wangjw(王家旺),huangyx(黄永秀).structuralcomparisonsofpolyhedrinproteinsandphylogenyofbaculaviruses.chinbiodiv(生物多样性),1997,5(1):14~25
|
| [7] | 7sunxl(孙修炼),zhanggy(张光裕).acomparisonoffourwildisolatesofheliothisnuclearpolyhedrosisvirus.virolsin(中国病毒学),1994,9(4):309~317
|
| [8] | 8sungx(孙国勋),qinql(秦启联),caixy(蔡秀玉),dingc(丁翠).aggregatepropertyofhanpvpolyhedrinandtheserologicalrelationshipamonginclusionbodyproteins.actaentomolsin(昆虫学报),2002,45(1):24~29
|
| [9] | 9sunxl(孙修炼),zhangzx(张忠信),zhanggy(张光裕).astainingmethodforcountingpolyhedrainnuclepolyhedrovirusinsecticide.chinjbiolcontrol(中国生物防治),1999,15(3):127~129
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
