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OALib Journal期刊
ISSN: 2333-9721
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亚麻温水脱胶液中细菌菌群结构分析
DOI: 10.3724/SP.J.1145.2009.00703, PP. 703-707
Keywords: 亚麻,细菌多样性,变性梯度凝胶电泳（dgge）,温水脱胶
Abstract:
为了探讨实验室条件下模拟的工厂亚麻温水脱胶液中细菌菌群结构，采用纯培养技术和pcr-dgge技术（denaturinggradientgelelectrophoresis）对细菌菌群结构进行了研究.用纯培养方法分离获得9类菌落，其中假单胞菌属（pseudomonas）在有氧培养条件下总是处于优势.梭菌属（clostridium）在厌氧培养过程中总是处于优势.微球菌属（micrococcus）和葡萄球菌属（staphylococcus）只有亚麻脱胶初期才有发现.pcr-dgge指纹图谱显示，亚麻温水脱胶过程中条带数量较少且没有明显种群群落结构演替过程.通过对不同时期沤麻液中16srdnav3片段pcr产物d、e两个dgge条带进行分子克隆、序列测定和blast分析，发现e条带包含的16srdnav3片段除e_35外均属于假单胞菌属.d条带包含着较多不同的16srdnav3片段，其中有传统方法没有分离到的泛菌属（pantoea）细菌、一些ncbi未收录的序列及一些非可培养微生物序列.纯培养技术和pcr-dgge技术的共同使用，可以更全面准确地提供细菌多样性方面的信息.图4参15
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